The SWI/SNF complex is an ATP-dependent chromatin remodeling complex that plays pivotal roles in gene regulation and cell cycle control. In the present study, we explored the molecular functions of the BAF57 subunit of SWI/SNF in cell cycle control via transcriptional regulation of cell cycle-related genes. We affinity purified SWI/SNF from HeLa cells stably expressing FLAG-tagged BAF47/Ini1 wi...

Common bean (Phaseolus vulgaris L.) fixes atmospheric nitrogen (N2) through symbiotic nitrogen fixation (SNF) at levels lower than other grain legume crops. An understanding of the genes and molecular mechanisms underlying SNF will enable more effective strategies for the genetic improvement of SNF traits in common bean. In this study, transcriptome profiling was used to identify genes and mole...

The microphthalmia transcription factor (Mitf) activates melanocyte-specific gene expression, is critical for survival and proliferation of melanocytes during development, and has been described as an oncogene in malignant melanoma. SWI/SNF complexes are ATP-dependent chromatin-remodeling enzymes that play a role in many developmental processes. To determine the requirement for SWI/SNF enzymes ...

The objective of this study was to determine the effect of different levels of sesame wastes (SW) on performance, milk composition and blood metabolites in lactating dairy cows. In this order, eight multiparous Holstein dairy cows were used in a replicated 4×4 Latin square design in four periods of 21 days. Treatments were control (no Sesame meal supplementat...

The objective of this study was to determine the effect of different levels of sesame wastes (SW) on performance, milk composition and blood metabolites in lactating dairy cows. In this order, eight multiparous Holstein dairy cows were used in a replicated 4×4 Latin square design in four periods of 21 days. Treatments were control (no Sesame meal supplementat...

Common bean (Phaseolus vulgaris L.) is the most important food legume, cultivated by small farmers and is usually exposed to unfavorable conditions with minimum use of inputs. Drought and low soil fertility, especially phosphorus and nitrogen (N) deficiencies, are major limitations to bean yield in smallholder systems. Beans can derive part of their required N from the atmosphere through symbio...

Drosophila SNF is a member of the U1A/U2B″/SNF protein family that is found in U1 and U2 snRNPs, where it binds to Stemloop II and Stemloop IV of U1 and U2 snRNA, respectively. SNF also binds to the U2A' protein, but only in the U2 snRNP. Although previous reports have implicated U2A' as a necessary auxiliary protein for the binding of SNF to Stemloop IV, there are no mechanisms that explain th...

We have previously shown that the yeast SWI/SNF complex stimulates in vitro transcription from chromatin templates in an ATP-dependent manner. SWI/SNF function in this regard requires the presence of an activator with which it can interact directly, linking activator recruitment of SWI/SNF to transcriptional stimulation. In this study, we determine the SWI/SNF subunits that mediate its interact...

Grain legumes are feedstuffs with high nutritional value that could replace soybean in dairy sheep nutrition. This be beneficial terms of economic efficiency and environmental sustainability. However, research regarding their potential effects on animal performance is scarce. The objective was to assess milk production body condition score (BCS) intensively reared ewes after replacing meal a mi...

Strong interest by Congress in a Medicare prospective payment system for skilled nursing facilities (SNF's) resulted in a major study by the Health Care Financing Administration on the Medicare SNF benefit. This article highlights findings from that study, which addressed the following: the Medicare SNF benefit, utilization and expenditures, the Medicare SNF industry, problems with the current ...