An agarose film has been proposed as an efficient substrate for producing microarrays. The original film preparation procedure was simplified significantly by grafting the agarose layer directly onto unmodified microscope glass slides instead of aminated glass slides, and the blocking procedure was replaced with a wash in 0.1x standard saline citrate (SSC) and 0.5% sodium dodecyl sulfate (SDS) ...

BACKGROUND Virtual slides are viewed using interactive software that enables the user to simulate the behaviour of a conventional optical microscope, like adjusting magnifications and navigating to any portion of the image. Nowadays, information about the performance and features of web-based solutions for reading slides in real environments is still scarce. The objective of this study is analy...

We demonstrate the feasibility of using qPCR on DNA extracted from vaginal Gram stain slides to estimate the presence and relative abundance of specific bacterial pathogens. We first tested Gram stained slides spiked with a mix of 10(8) cfu/ml of Escherichia coli and 10(5) cfu/ml of Lactobacillus acidophilus. Primers were designed for amplification of total and species-specific bacterial DNA ba...

Virtual slides are high-magnification digital images of tissue sections, stored in a multi-resolution file format. Using appropriate software, these slides can be viewed in a web browser in a manner that closely simulates examination of glass slides with a real microscope. We describe the successful implementation of teaching microscopic pathology with virtual slides and, for the first time, th...

CDISC Webinar: Learning Health Community ESTEL Update Initiative (Slides [1], Audio Recording [2]) (5 Nov 2014) CDISC Public Webinar Series Good Practice in PMA Submissions for Efficient Regulatory Decision Making (Slides [3], Audio Recording [4]) (21 Aug 2014) CDISC Public Webinar Series Introduction to CDISC Online Courses; (Slides [5], Audio Recording [6]) (5 June 2014); Update 9 June 2014: ...

Ramón y Cajal's studies in the field of neuroscience provoked a radical change in the course of its history. For this reason he is considered as the father of modern neuroscience. Some of his original preparations are housed at the Cajal Museum (Cajal Institute, CSIC, Madrid, Spain). In this article, we catalogue and analyse more than 4,500 of Cajal's histological preparations, the same prepara...

1 Likelihood Ratio Test Let X1, ..., Xn be a random sample from a distribution with pdf f(x|θ) where θ is some one dimensional (unknown) parameter. Suppose we want to test the null hypothesis, H0, that θ = θ0 against the alternative hypothesis, Ha, that θ = θ0. Assume the same regularity conditions hold as in the MLE theory. Then likelihood ratio test (LRT) statistic is λ(x) = L(θ0|x) L(θ̂ML|x) ...

A novel route was introduced to synthesize dense polyacrylamide (PAM) onto the glass slide surface. To investigate the surface chemistry of the PAM on the glass slides, X-ray photoelectron spectroscopy (XPS) was utilized to obtain detailed chemical state information on the PAM layer constituents. The XPS peak data were consistent with the presented model of the PAM on the glass slide surface. S...

Computerized presentation slides have become essential for effective business meetings, classroom discussions, and even general events and occasions. With the exploding number of online resources and materials, locating the slides of high quality is a daunting challenge. In this study, we present a new, comprehensive framework of information quality developed specifically for computerized prese...

In a 3 X 2 X 2 factorial design with repeated measures on the last factor, a suicide attempt, suicide ideation, and nonsuicidal control group of Veterans Administration neuropsychiatric patients, equally divided into psychotic and neurotic subgroups, containing IS Ss each, viewed 2 classes of slides—unpleasant slides and death slides. Measures of viewing time, rated tension, and rated liking we...