The aim of the present study was to develop a genetic sex marker for the pointed gourd (Trichosanthes dioica Roxb.) to allow gender determination at any stage in the life cycle. Screening of genomic DNA with intersimple sequence repeat (ISSR) primers was used to discover sex-specific touch-down polymerase chain reaction (Td-PCR) amplification products. Using pooled DNA from male and female geno...

the genetic diversity of 89 flue-cured tobacco varieties was examined using 12 issr primers. these cultivarswere evaluated at the guilan tobacco research center, rasht, iran, using a 7×7 simple lattice design with two replications, and 12 morphological traits. the total number of pcr amplified products was 143 bands ranging from 450 to 3000 bp, of which 108 bands (74.28%) were polymorphic. prim...

Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and population structure of M. truncata base...

Better knowledge of medicinal plant species and their conservation is an urgent need worldwide. Decision making for conservation strategies can be based on the knowledge of the variability and population genetic structure of the species and on the events that may influence these genetic parameters. Achyrocline flaccida (Weinm.) DC. is a native plant from the grassy fields of South America with ...

Houttuynia cordata is an important traditional Chinese herb with unresolved genetics and taxonomy, which lead to potential problems in the conservation and utilization of the resource. Inter-simple sequence repeat (ISSR) markers were used to assess the level and distribution of genetic diversity in 226 individuals from 15 populations of H. cordata in China. ISSR analysis revealed low genetic va...

Pearl millet (Pennisetum glaucum (L.) R. Br.) and napier grass (Pennisetum purpureum Schumach) are the most economically important species in the genus Pennisetum. Knowledge of genetic diversity of these two species would enhance their potentials for utilization. The present work assessed the genetic diversity and phylogenetic relationship among Nigerian and Indian accessions of pearl millet an...

The utility of inter simple sequence repeat-PCR (ISSR-PCR) assay in the genetic characterisation and elucidation of the phylogenetic relationship of different microsporidian isolates infecting tropical tasar silkworm, Antheraea mylitta Drury, is demonstrated. A total of 22 different microsporidians collected from the diseased tasar silkworms from Jharkhand state of India were analysed using mor...

In a survey of root-knot nematodes in Iran, twenty-nine populations were collected from eleven provinces. All identified as Meloidogyne javanica based on morphological and morphometric characteristics females, males, J2s, perineal patterns. Sequence Characterized Amplified Region (SCAR) primers confirmed the studies, all these produced specific band 670 bp using Fjav Rjav primers. Genetic struc...