Here, we report the synthesis of 3,6,9-trioxaundecan-1-{4-[(2-Chloroethyl)Ethylamino)]-Benzylamino},11-Azide (CEBA). CEBA alkylates N7 guanine DNA thanks its chloroethyl group and can be coupled by a strain-promoted azide-alkyne cycloaddition to an alkynylated molecule. The optimization alkylation level pDNA reveals that expression encoded gene is preserved when it randomly modified with at mos...

We report fluorescence imaging of single dye molecules on a glass substrate by two-photon excitation with femtosecond pulses from a mode-locked Ti:sapphire laser. The single-molecule images exhibit a high signal to background ratio (>30:1) and a high spatial resolution (fwhm < /3). A quadratic intensity dependence of single-molecule emission rate is experimentally verified, and single-molecule ...

The non-radiative coupling of a molecule to a metallic spherical particle is approximated by a sum involving particle quasistatic polarizabilities. We demonstrate that energy transfer from molecule to particle satisfies the optical theorem if size effects corrections are properly introduced into the quasistatic polarizabilities. We hope that this simplified model gives valuable information on t...

Circularly polarized luminescence (CPL) and Raman optical activity (ROA) were observed in a single spectroscopic experiment for purely organic molecule, an event that had so far been limited to lanthanide-based complexes. The present observation was achieved [16]cycloparaphenylene lemniscate, double macrocycle constrained by rigid 9,9′-bicarbazole subunit, which introduces chirality source allo...

in this study, polyaniline molecule (emeraldine base) is modeled as a molecular wire and the effects of the metal/molecule coupling strength and the molecule length on the current-voltage (i-v) characteristics are numerically investigated. using a tight-binding hamiltonian model, the methods based on non-equilibrium green’s function theory, landauer formalism and newns-anderson model, our calcu...

Major sperm protein, a cytoskeletal molecule required for the amoeboid motility of sperm in Caenorhabditis elegans, also functions as a signaling molecule that regulates the rates of meiotic maturation and ovulation. Recent work has begun to uncover new genes required for the response to this signal in both somatic and germ line cells.

Methods for the detection of specific interactions between diverse proteins and various small-molecule ligands are of significant importance in understanding the mechanisms of many critical physiological processes of organisms. The techniques also represent a major avenue to drug screening, molecular diagnostics, and public safety monitoring. Terminal protection assay of small molecule-linked D...

Regulation of the direction of flagellar rotation is central to the mechanism of bacterial chemotaxis. The transitions between counterclockwise and clockwise rotation are controlled by a "switch complex" composed of three proteins (FliG, FliM, and FliN) and located at the base of the flagellar motor. The mechanism of function of the switch is unknown. Here we demonstrate that the diffusible clo...