Bacillus subtilis harbors seven extracytoplasmic function (ECF) sigma factors. At least three ECF sigma factors (sigma(M), sigma(W), and sigma(X)) are induced by, and provide resistance to, antibiotics and other agents eliciting cell envelope stress. Here, we report that ECF sigma factors also contribute to antibiotic production. B. subtilis 168 strains that are lysogenic for the SPbeta bacteri...

We used limited trypsin digestion to determine the domain organization of the Escherichia coli RNA polymerase sigma 70 subunit. Trypsin-resistant fragments containing sigma 70 conserved region 2 (sigma 70(2)), and carboxy-terminal fragments containing conserved regions 3 and 4 (sigma 70(3-4)) were identified by a combination of amino acid sequencing and mass spectrometry. The domains were studi...

Upon environmental changes, bacteria reschedule gene expression by directing alternative sigma factors to core RNA polymerase (RNAP). This sigma factor switch is achieved by regulating relative amounts of alternative sigmas and by decreasing the competitiveness of the dominant housekeeping sigma(70). Here we report that during stationary phase, the unorthodox Crl regulator supports a specific s...

Rhizobia are soil bacteria able to establish a nitrogen-fixing symbiosis with legume plants. Both in soil and in planta, rhizobia spend non-growing periods resembling the stationary phase of in vitro-cultured bacteria. The primary objective of this work was to better characterize gene regulation in this biologically relevant growth stage in Sinorhizobium meliloti. By a tap-tag/mass spectrometry...

The sigma-receptor, a broadly distributed integral membrane protein with a novel structure, is known to modulate various voltage-gated K(+) and Ca(2+) channels through a mechanism that involves neither G proteins nor phosphorylation. The present study investigated the modulation of the heart voltage-gated Na(+) channel (Na(v)1.5) by sigma-receptors. The sigma(1)-receptor ligands [SKF-10047 and ...

 In Proposition 2.6 in (G‎. ‎Gruenhage‎, ‎A‎. ‎Lutzer‎, ‎Baire and Volterra spaces‎, ‎textit{Proc‎. ‎Amer‎. ‎Math‎. ‎Soc.} {128} (2000)‎, ‎no‎. ‎10‎, ‎3115--3124) a condition that‎ ‎every point of $D$ is $G_delta$ in $X$ was overlooked‎. ‎So we‎ ‎proved some conditions by which a Baire space is equivalent to a‎ ‎Volterra space‎. ‎In this note we show that if $X$ is a‎ ‎monotonically normal $T_1...

RATIONALE, AIMS AND OBJECTIVES Timely communication is important to ensure high-quality health care. To facilitate this, the Gastro Intestinal Oncology Center Amsterdam (GIOCA) stipulated to dispatch medical reports on the day of the patient's visit. However, with the increasing number of patients, administrative processes at GIOCA were under pressure, and this standard was not met for the majo...

Bacterial sigma (sigma) factors are an essential component of RNA polymerase and determine promoter selectivity. The substitution of one sigma factor for another can redirect some or all of the RNA polymerase in a cell to activate the transcription of genes that would otherwise be silent. As a class, alternative sigma factors play key roles in coordinating gene transcription during various stre...

Cruz Biotechnology), anti-FLAG (M2; Sigma Chemical Co., St. Louis, MO), anti-β-actin (AC-15; Sigma), and anti-α-tubulin (B-5-1-2; Sigma Chemical Co.) were used. Anti-Synoviolin monoclonal antibody (10Da) was reported previously (Amano et al., 2003). Cycloheximide (Sigma Chemical Co., 100μg/mL) was used for the chase experiment of p53. Camptothecine (Sigma, 0.5 mM), and actinomycin D (Sigma Chem...