Real-time PCR-based molecular diagnostic techniques were used for the comparative identification of the most common KRAS and BRAF mutations from methanol liquid-based cytology samples and matched, formalin-fixed and paraffin-embedded tissue samples. Methanol-based fixation and preservation of brushing samples of colorectal carcinomas could contribute to sample collection, short-term storage and...

In this study we compared the effects of duration of cold ischemia (longer and shorter ischemia) on the yield, viability and preservation of the physiological function and insulin secretion of adult human pancreatic islets in short-term (seven days) culture. Based on the tested parameters, we established that there are no major differences between these two test groups and that the storage and ...

We propose a new, logical, approach to the decidability problem for the Straubing and Brzozowski hierarchies based on the preservation theorems from model theory, on a theorem of Higman, and on the Rabin tree theorem. In this way, we get purely logical, short proofs for some known facts on decidability, which might be of methodological interest. Our approach is also applicable to some other sim...

EXECUTIVE SUMMARY Having confidence in the permanence of a digital resource requires a deep understanding of the preservation activities that will need to be performed throughout its lifetime, and an ability to plan and resource for those activities. The LIFE (Lifecycle Information for E-Literature) Project has advanced understanding of the short and long-term costs in this complex area, facili...

Abstract All the cards seem to be stacked against belief in immortality. Nonetheless, resources of particular religious traditions may avail where generic philosophical solutions fall short. With attention boredom and narcissism critiques, intimations deathlessness Śāntideva's radical altruism, recent Christian debates on soul intermediate state, I propose two criteria for a coherent religion-s...

This paper gives an overview over different photogrammetric single image techniques, like digital rectification, unwrapping of parametric surfaces and differential rectification methods. It shows the new possibilities for the generation of orthoimages and image mosaics using digital image processing methods even on low-cost personal computers. After a short description of the implemented method...

During the organ transplantation process, conservation solutions must address responses to the physiologic organ preservation and prevent ischemia-reperfusion injuries. The use of colloids seems beneficial especially for long ischemia time compared to the impermeant molecules used for short time. The colloids family includes molecules as hydroxyethyl starch (HES), albumin, dextran or polyethyle...

objective: the present study aims to verify web preservation projects and programs in order to explore their preferred solutions in tackling the technical challenges.   method: a documentary method was used to identify and analyze web-preservation-related literature, including papers, handbooks, web sites and documents. the programs’ persons in charge were also questioned. besides, top web pres...

Conventional methods for the propagation and preservation of parasites in vivo or in vitro have limitations, which include the need for labor, the initial isolation and loss of strains, bacterial and fungal contamination, and changes in the original biological and metabolic characteristics of the organism. All of these disadvantages are reduced considerably by cryopreservation. In this study, w...

Preservation of information is not a new issue but preservation of digital information has a relatively short history. Since the 60’s when computers began to be used within administration, digital information that has had to be preserved over time. The problem addressed in this research is how to preserve understandable information over time. Information is context dependent, which means that w...