Bacterial identification relies primarily on culture-based methodologies requiring 24h for isolation and an additional 24-48h for speciation. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is an emerging technology newly applied to the problem of bacterial speciation. We evaluated two MALDI-TOF MS systems (MS) on 720 consecutively isolated bacterial ...

BACKGROUND Selenium, like other trace elements and antioxidant enzymes, is known as an antioxidant and immunomodulator trace element. Due to recent evidence for selenium deficiency in Behcet's syndrome, this study is to evaluate the correlation of serum selenium level with Behcet's disease (BD). MATERIALS This case-control study was conducted on 46 BD patients and 46 healthy controls in a rhe...

In order to perform experimental identification of high strain rate material models, engineers have only a very limited toolbox based on test procedures developed decades ago. The best example is the so-called split Hopkinson pressure bar based on the bar concept introduced 100 years ago by Bertram Hopkinson to measure blast pulses. The recent advent of full-field deformation measurements using...

Chemically stable ester derivatives of vitamins A, C and E have become a focus of interest for their role in the satisfactory results in skin aging treatments. Accordingly, the aim of this study was to evaluate the physical and chemical stability of a cosmetic formulation containing 1% retinyl palmitate, ascorbyl tetraisopalmitate and tocopheryl acetate, alone or in combination. In the studies ...

Suppositories are important tools for individual therapy, especially in paediatrics, and an instrumental assay method has become necessary for the quality control of dosage units. The aim of this work was to develop a rapid, effective high-performance liquid chromatography method to assay aminophenazone in extemporaneous suppositories prepared with two different suppository bases, adeps solidus...

The effect of 8-methoxypsoralen-UVA therapy on the catalysis of histidine to trans-urocanic acid by histidine ammonia lyase (HAL, EC 4.3.1.3) was examined using an enzymatic assay from Sigma-Aldrich where the growth of the trans-urocanic acid peak at 277 nm was monitored. A Rayonet Photochemical Mini Reactor (Model RMR-600) equipped with eight, 3500 Å light sources and a custom UVA filter (Mode...

INTRODUCTION Small scale and large scale farmers around Lusaka, the capital city of Zambia grow vegetables using intensive agriculture methods to satisfy the ever increasing demand. To ensure maximum yield they apply various types of pesticides to control pests and diseases that attack these vegetables. Organophosphate pesticides are widely used in agriculture for the control of various insect ...

Electrophoretic separation techniques using microchips have been dramatically improved and several commercial MCE systems have been developed. For example, the Agilent Technologies 2100 Bioanalyzer system is commercially available with a protein separation chip and reagent kit that enables rapid and highly efficient protein separation based on their molecular size [1]. Separation of protein mix...

A stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of Bendamustine hydrochloride in injectables. Reversed-phase chromatography was performed on Shimadzu Model CBM20A/20 Alite, equipped with SPD M20A prominence photodiode array detector using C18 (250 mm × 4.6 mm, 5 μm particle size) column with acetonitrile: tetra butyl ammoni...

ionization mass spectrometry method is developed and validated for the identification and quantitation of azithromycin in human plasma. After the addition of the internal standard and 1.0M sodium hydroxide solution, plasma samples are extracted with a methylene chloride–ethyl acetate mixture (20:80, v/v). The organic layer is evaporated under a stream of nitrogen at 40°C. The residue is reconst...