Two Citrus sinensis (L.) phosphomannose isomerase (PMI) genes, CsPMI1 and CsPMI2, were evaluated as novel selectable markers in mature citrus transformation. Transgenic shoots produced after transformation of Kuharske rootstock with each PMI construct selected on six treatments mannose sucrose. For CsPMI1, there no significant differences among the various sucrose for mean number positive (PS),...

The orange tree (Citrus sinensis) is a species of fruit shrubs the Rutaceae family. Essential oils from citrus peels are widely used in various agro-food and pharmaceutical preparations hence they must be recovered using green extraction methods. In this study, we compare efficiency five techniques (conventional hydro-distillation (HD), Salt-assisted by HD (S-HD), Ultrasound-assisted (US-HD), E...

Papain-like cysteine proteases (PLCPs) play important roles in plant-pathogen interactions. In citrus, PLCPs are targeted by effectors from Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing (HLB). To study interactions between Citrus sinensis and CLas, we identified 33 candidate CsPLCP genes HMMsearch with Peptidase_C1 domain (PF00112), further confirmed them using loc...

background: application of plant extracts as mosquito control strategy was practiced from centuries. these are easily available, non-toxic, biodegradable and exhibit broad-spectrum target specific activities against larval stages of mosquitoes. method: different potential parts of locally grown plants, seeds of nutmeg ( myristica fragrans ), peel of musambi ( citrus sinensis ), leaves of babuna...

Phyllocnistis citrella Stainton, the citrus-leafminer, is an important pest of citrus worldwide. Knowledge of natural parasitism levels is fundamental to the establishment of tactics of management and control of this species. This work aimed to evaluate the parasitism in two citrus orchards, one of ‘Montenegrina’ (Citrus deliciosa Ten.) and the other of ‘Murcott’ (C. sinensis (L.) Osbeck x C. r...

larvae of dicrodiplosis manihoti harris (dip., cecidomyiidae) are the predator of mealybug ovisacs (hem.: pseudococcidae) in fars province, iran. parasitized larvae of this predator were collected from nipaecoccus viridis (newstead) on citrus sinensis (l.) and reared in the laboratory. parasitoid wasps belonging to the family torymidae were consequently collected, and identified as pseudotorymu...

The infectivity of five populations of Tylenchulus semipenetrans were compared and differentiated on 10 hosts (5 Citrus spp., 1 Poncirus trifoliata, and 4 hybrids of Citrus spp. X P. trifoliata). Differences in levels of infection and development (P = 0.01) occurred between Citrus spp. and P. trifoliata cv. 'Pomeroy' and their three hybrids, C. paradisi X P. trifoliata cv. 'Swingle' citruntelo ...

Free radicals play an important role in stomach ulcer formation. The present investigation validates the anti ulcer activity of hesperidin, isolated from Citrus sinensis (L.) Osbeck, Rutaceae, through the assessment of its antioxidant potential over stomach mucosal tissue by histological examination. Hesperidin was isolated from the dried peel of C. sinensis, and authenticated by TLC, IR and HP...

TERMINAL FLOWER is a key regulator of floral timing in Arabidopsis and other herbaceous species. A homolog of this gene, CsTFL, was isolated from the hybrid perennial tree crop Washington navel orange (Citrus sinensis L. Osbeck). The deduced amino acid sequence of CsTFL was 65% identical to the Arabidopsis TFL1 protein. Wild-type Arabidopsis plants ectopically expressing CsTFL showed late-flowe...

Most of the plant regeneration processes in citrus, through tissue culture, involve indirect somatic embryogenesis. The optimization of these processes is important for the development of in vitro plant improvement and micropropagation studies. Studies to evaluate the effect of different carbohydrates in somatic embryogenesis were conducted using calli from ‘Ponkan’ mandarin (Citrus reticulata,...