Internalization of PAK strain Pseudomonas aeruginosa into human respiratory epithelial cell lines and HeLa cervical cancer cells in vitro was readily demonstrable via a gentamycin protection assay. Depletion of target cell glycosphingolipids (GSLs) using a glucosyl ceramide synthase inhibitor, P4, completely prevented P. aeruginosa internalization. In contrast, P4 treatment had no effect on the...

Objective: To investigate infection distribution of Pseudomonas aeruginosa on burned patients in our hospital as well as its drug resistance in order to provide reference of clinical use of antibiotics. Methods: Statistically analysed 481 Pseudomonas aeruginosa and their drug sensitive test results from burned patients who were sent to hospital from January 2004 to December 2006. Results: In ou...

Eighty-eight Pseudomonas aeruginosa isolates, most of them from the Collection of Bacterial Strains of the Institut Pasteur, Paris, were analysed for their pyoverdine-mediated iron incorporation system by different methods, including pyoverdine isoelectrofocusing analysis, pyoverdine-mediated growth stimulation, immunoblot detection of (ferri)pyoverdine outer-membrane receptor and pyoverdine-fa...

Microcystis aeruginosa is a freshwater bloom-forming cyanobacterium that is distributed worldwide. M. aeruginosa can be divided into at least 8 phylogenetic groups (A-G and X) at the intraspecific level. Here, we report the complete genome sequence of M. aeruginosa NIES-2481, which was isolated from Lake Kasumigaura, Japan, and is assigned to group G. The complete genome sequence of M. aerugino...

Decreased permeability to imipenem is the most frequent mechanism of imipenem resistance in Pseudomonas aeruginosa. We have determined the presence of OprD porin protein, an imipenem influx channel, in 70 carbapenem-resistant P. aeruginosa clinical isolates by Western blot analysis using rabbit anti-OprD polyclonal antibody. Ninety-eight percent (54 of 55 isolates) of imipenem-and meropenem-res...

Despite routine monitoring and disinfection, treated swimming pools are frequently contaminated with the opportunistic pathogen Pseudomonas aeruginosa, which can represent a significant public health threat. This review was undertaken to identify the current understanding of risk factors associated with pool operation with respect to P. aeruginosa. The ecology and factors that promote growth of...

conclusions the findings of the present study well demonstrated that the p. aeruginosa biofilm-producing isolates were more resistant to the tested antibiotics. furthermore, because of wide distribution, it seems that the psla gene is associated with biofilm formation. results biofilm formation was observed in 70% of the p. aeruginosa isolates. the potential formation of biofilm was significant...

background : bacterial infections in burn and wound patients are common and difficult to control. the aim of the current study was to evaluate the ability of full length oprf to elicit the production of protective igg in mice burn wound sepsis model against p. aeruginosa infection. methods : oprf protein was expressed and purified by ni-nta. the purified protein as used to immunize balb/c mice....

multidrug resistant (mdr) bacteria are spread through the world which causes nosocomial infections, especially in intensive care unit (icu). this study aimed to investigate resistance pattern of pseudomonas aeruginosa and enterobacter cloacae isolated from patients in icu. during 2011-2012, 30 isolates for each p. aeruginosa and e. cloacae were collected from patients who acquired nosocomial in...

Pseudomonas aeruginosa is an opportunistic pathogen causing severe infections often characterized by robust neutrophilic infiltration. Neutrophils provide the first line of defense against P. aeruginosa. Aside from their defense conferred by phagocytic activity, neutrophils also release neutrophil extracellular traps (NETs) to immobilize bacteria. Although NET formation is an important antimicr...