نتایج جستجو برای: serum free medium
تعداد نتایج: 1005507 فیلتر نتایج به سال:
The influence of serum on the synthesis of the nervous system specific S-100 protein has been investigated in the rat glial cell clone C61a. In sparse cells, where S-100 synthesis is low, removal of serum leads to a 2- to 3-fold increase of the rate of S-100 synthesis relative to total soluble cell protein. Measurements of S-100 content by radioimmunoassay showed that sparse cells are accumulat...
OBJECTIVE To determine if insulinlike growth factor I (IGF-I) and basic fibroblast growth factor (bFGF), individually or in combination, support the growth and viability of human septal chondrocytes in a serum-free medium (SFM) and a serum-enhanced culture medium. DESIGN Chondrocytes were recovered from enzymatically digested human septal cartilage and were plated for monolayer culture in a n...
It was previously reported from this laboratory that the secondary antibody response can regularly be elicited in vitro from fragments of rabbit lymph node node cultured in Eagle's medium supplemented with normal rabbit serum. Evidence is now presented that physiological levels of hydrocortisone (0.01 to 1.0 microM) can substitute for serum in the culture medium. However, with the omission of s...
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