نتایج جستجو برای: separation and purification

تعداد نتایج: 16849632  

2014
Derek McVay G. S. Samuelsen

Polygeneration of hydrogen, heat, and power has been shown to improve overall plant efficiency by creating more products and introducing synergy which improve fuel cell performance (Figure 1). The integration of an absorption chiller with a high temperature fuel cell for polygeneration of combined cooling, heating, hydrogen, and power is studied. Steady-state and dynamic computer models are uti...

Journal: :Fermentation 2022

In this study, the fermentation broth of recombinant Pichia pastoris strain ncy-2 was studied. After pretreatment, separation, and purification, lysozyme optimized using biofilm ion exchange separation. Finally, dry enzyme powder prepared by concentrating vacuum drying. The removal rate bacterial cells 99.99% when centrifuged at low temperature. optimum conditions were: transmembrane pressure 0...

Journal: :Cancer research 1988
J R Yannelli G B Thurman A Mrowca-Bastin C S Pennington W H West R K Oldham

In a continued effort to make interleukin-2/lymphocyte-activated killer (LAK) cell therapy safer and more efficacious for cancer patients, we examined methods of increasing the yields of cells obtained as a final product for reinfusion. Previously, the major cell loss occurred in the Ficoll-Hypaque gradient separation procedure used before cell culture. Therefore, we investigated the necessity ...

Journal: :The Journal of biological chemistry 1968
E T Yin S Wessler

Parke-Davis bovine thrombin was resolved into three distinct protein peaks on a Sephadex G-200 column. A stepwise chromatographic technique on diethylaminoethyl (DEAE) cellulose was developed for the complete separation and partial purification of thrombin and activated Factor X. Thrombin was quantitatively eluted in the first protein peak with 0.1 M NaCl, pH 7.0. A second peak with residual th...

Introduction: Separation of molecules and cells using magnetic force is very simple, fast, efficient and low cost method in medical laboratory and research activities. The usage of this separation contributes to the quality of studies and related calculations in the field of laboratory sciences and researches. Methods and Materials: In the present study, we managed to make an optimal device fo...

Polyclonal antibodies against kappa light chain are used to diagnose diseases producing free light chain. The kappa and lambda light chains are products of immunoglobulin synthesis and released into the circulation in minor amounts such as serum, cerebrospinal fluid, urine and synovial fluid in normal condition. The purpose of this study was the production and purification of polyclonal immunog...

A Mohseni Meybodi F Sangsefidi M Sabbaghian, MA Sadighi Gilani, P Mokhtari

Background Sertoli cells located in seminiferous tubular basement membrane and surrounds different types of germ cell and they were only somatic cell type that directly contacts germ cells. These cells have Several role like support activities, nutrition of germ cells.During spermatogenesis, expression of SOX3 is necessary for differentiation of spermatogonia A. SOX3 is a transcription factor, ...

2016
M. Castaldo L. Barlind F. Mauritzson P. T. Wan H. J. Snijder

Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method where affinity resin is contained within porous-walled containers, supporting clarification, produ...

Journal: :Applied and environmental microbiology 2005
Gavin C Barnard Jesse D McCool David W Wood Tillman U Gerngross

Protein purification of recombinant proteins constitutes a significant cost of biomanufacturing and various efforts have been directed at developing more efficient purification methods. We describe a protein purification scheme wherein Ralstonia eutropha is used to produce its own "affinity matrix," thereby eliminating the need for external chromatographic purification steps. This approach is b...

2014
Gang Xu Feifei Liang Yongping Yang Wenyi Liu

In this study, an improved CO2 separation and purification system is proposed based on in-depth analyses of cryogenic separation and distillation theory as well as the phase transition characteristics of gas mixtures containing CO2. Multi-stage compression, refrigeration, and separation are adopted to separate the majority of the CO2 from the gas mixture with relatively low energy penalty and h...

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