The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1). Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within t...

Group II intron ribozymes fold into their native structure by a unique stepwise process that involves an initial slow compaction followed by fast formation of the native state in a Mg(2+)-dependent manner. Single-molecule fluorescence reveals three distinct on-pathway conformations in dynamic equilibrium connected by relatively small activation barriers. From a most stable near-native state, th...

Please cite this article in press as: Atif, Y. Conve http://dx.doi.org/10.1016/j.chb.2012.07.026 Today’s college students have grown up with technology. These digital natives typically gravitate toward group activities in technology embedded social contexts. However, despite this multidimensional evolution, little has changed in conventional classrooms where they build their education experienc...

This chapter analyzes the adequacy of two university education models according to their Information Communications Technology (ICT) usage: One model is based on the use of ICT as a cornerstone and a cohesive element of the teaching-learning process, whereas the other considers ICT a mere teaching support tool. The preliminary results obtained from a review of case studies reveal that ICT use i...

We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiati...

Time-resolved fluorescence of 4',6-diamidino-2-phenylindole (DAPI) complexes show that for a homogeneous polymer (polyd(AT) or polyd(A).polyd(T)) at high P/D (phosphate/dye) ratio, a single exponential component adequately describes the fluorescence decay. For the AT polymers at low P/D ratio or for native DNA, the decay cannot be described by a single-exponential term. A continuous distributio...

Fluorescent analogues of the natural DNA bases are useful in the study of nucleic acids' structure and dynamics. 2-Aminopurine (2AP) is a widely used analogue with environmentally sensitive fluorescence behavior. The quantum yield of 2AP has been found to be significantly decreased when engaged in π-stacking interactions with the native bases. We present a theoretical study on fluorescence quen...

The fluorescence characteristics of norfloxacin (NFLX) were studied in reversed micelles which formed with sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-water-octane. The influences of the environmental factors, such as the water content, AOT concentration and pH, on the fluorescence of NFLX were investigated. A novel spectrofluorimetric method for the direct determination of NFLX is proposed b...