نتایج جستجو برای: sensitized chemiluminescence

تعداد نتایج: 22524  

Journal: :Fertility and sterility 2009
Reda Mahfouz Rakesh Sharma Jakob Lackner Nabil Aziz Ashok Agarwal

OBJECTIVE To examine simultaneously the levels of hydrogen peroxide (H(2)O(2)) and superoxide (O(2)(-*)) using chemiluminescence and flow cytometry. DESIGN Prospective laboratory study. SETTING Reproductive research lab in a tertiary hospital. PATIENT(S) Semen samples from 18 healthy male volunteers. INTERVENTION(S) Sperm preparation and measurement of reactive oxygen species (ROS) by c...

Journal: :Gut 1994
G R Davies N J Simmonds T R Stevens M T Sheaff N Banatvala I F Laurenson D R Blake D S Rampton

To determine if reactive oxygen metabolites have a pathogenic role in Helicobacter pylori (H pylori) related gastroduodenal disease, this study measured their production in antral mucosal biopsy specimens. Two related chemiluminescence techniques were used comparing H pylori positive (n = 105) and negative patients (n = 64) with a similar spectrum of macroscopic disease. After chemiluminescence...

Journal: :Clinical chemistry 1996
I Bronstein C S Martin J J Fortin C E Olesen J C Voyta

A series of enzyme-activated chemiluminescence-based assays of reporter gene expression, useful in many biomedical applications, has been developed. The chemiluminescence detection systems for beta-galactosidase, beta-glucuronidase (GUS), and secreted placental alkaline phosphatase (SEAP) reporter enzymes are all based on use of 1,2-dioxetane substrates. This detection technology also permits t...

2011
Juozas žilinskas Jonas žekonis Gediminas žekonis Renata Šadzevičienė Marija Sapragonienė Justina Navickaitė Ingrida Barzdžiukaitė

BACKGROUND The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis. MATERIAL/METHODS The study was performed using veno...

Journal: :The Analyst 1998
C Molins-Legua P Campíns-Falcó A Sevillano-Cabeza

On-line automation of two different liquid chromatographic procedures, a pre-column derivatization system and a pre- and post-column system, in order to generate chemiluminescence is reported. Dansyl chloride (Dns-Cl) was used as a pre-column reagent to form fluorophores and bis(2,4,6-trichlorophenyl) oxalate (TCPO) and hydrogen peroxide (H2O2) as a post-column reagent to generate chemiluminesc...

Journal: :Blood 1984
G W Sullivan G R Donowitz J A Sullivan G L Mandell

Stimulated neutrophils show ionic fluxes that may function as "transducers" between stimuli and effector functions. Using fluorescent probes, patterns of membrane-associated calcium (chlortetracycline, CTC) and membrane potential (3-3'-dipentyloxacarbocyanine, di-O-C5 (3)) in single living human neutrophils were observed with a fluorescence microscope fitted with an image intensifier and photom...

Journal: :Biological research 2004
Laura B Valdez Silvia Alvarez Tamara Zaobornyj Alberto Boveris

The antioxidant capacity of polyphenols (+)-catechin, (-)-epicatechin and myricetin, and of different types of red wines (Cabernet Sauvignon, Malbec and blended wine) was evaluated by three assays. (a) NADH oxidation by peroxynitrite (ONOO-): the ONOO- scavenging activity was higher for myricetin (IC50=35 microM) than for (+)-catechin (IC50=275 microM) and (-)-epicatechin (IC50=313 microM). (b)...

Journal: :journal of nanostructures 2014
m. mazloum ardakani a. dehghani firouzabadi a. benvidi b. b fatemeh mirjalili r. zare

new kinds of hydroquinone derivatives were synthesized and along with a azo dye applied as additives in the iodide/iodine redox electrolyte for dye-sensitized solar cells and their effect on the short-circuit photocurrent of dye sensitized solar cells was investigated. addition of 0.05 m a hydroquinone derivative in the electrolyte comprising 0.5 m 1-methyl-3-propylimidazolium iodide (mpii) and...

Journal: :iranian journal of allergy, asthma and immunology 0
mohamed abdel-aziz biotechnology research unit, gastro-enterology center, mansoura university, mansoura, egypt. ayman abass biotechnology research unit, gastro-enterology center, mansoura university, mansoura, egypt and king fahd medical research center, king abdulaziz university, p.o. box, 80216 jeddah 21589 ,kingdom of saudi arabia. khaled zalata pathology departments ,faculty of medicine, mansoura university, mansoura, egypt. tarek abd al-galel pediatric allergy and immunology departments ,faculty of medicine, mansoura university, mansoura, egypt. umamma allam king fahd medical research center, king abdulaziz university, p.o. box, 80216 jeddah 21589 ,kingdom of saudi arabia. gamal karrouf king fahd medical research center, king abdulaziz university, p.o. box, 80216 jeddah 21589 ,kingdom of saudi arabia and department of surgery, anesthesiology & radiology, faculty of veterinary medicine, mansoura university, mansoura, 35516 dakahlia, egypt. gkarrouf@kau.edu.sa.

the aim of this study was to investigate the effects of nigella sativa  (ns) fixed oil in comparison to dexamethasone (dex) on inducible nitric oxide synthase (inos), peripheral blood eosinophils (pbe), allergen specific serum igg1 and interleukins and airway inflammation in a murine model of allergic asthma. thirty-one mice were divided into four groups. group  i (n =  6) served as the control...

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