The effect of Brilliant Green on motility was studied with Salmonella anatum, S. derby, S. tennessee, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa. Semisolid tryptic soy-agars containing 0, 20, or 40 mg of Brilliant Green per liter were used as the motility media. Both concentrations of Brilliant Green inhibited the growth of the non-Salmonella species through the semisolid ag...

T Slow cooling and long stirring produce particles that are large and spherical, while fast cooling and short stirring produce small, jagged particles that tend to stick together. As a result, the larger particles slide by each other more easily than the smaller ones do, producing a semisolid with less resistance to flow. High-speed videos show that the semisolid advances smoothly and evenly, p...

Compliant fish skin is effectively in reducing drag, thus the design and application of compliant bionic materials may be a good choice for drag reduction. Here we consider the drag reduction of compliant bionic materials. First, ZnO and PDMS mesh modified with n-octadecane were prepared, the drag reduction of self-texturing compliant n-octadecane were studied. The results show that the mesh mo...

Serum bactericidal assays (SBAs) for Group B meningococci are considered the methods of choice for the evaluation of functional antimeningococcal antibodies. Many investigators regard SBAs as time- and labor-intensive. Variations in SBA protocols among different laboratories make interpretation of results difficult. Here we describe a fluorescence-based serum bactericidal assay (fSBA) and compa...

A total of 230 cultures of fungi and 43 cultures of bacteria, isolated from such sources as soil, butter, and milk, were screened for their milk-clotting activity. The fungi were cultivated on semisolid media, and the bacteria were grown in milk media in shake culture. Phytic acid, added as calcium phytate, was found to stimulate production of the enzyme in most of the bacterial isolates. Prote...

Plesiomonas shigelloides is the unique member of the Enterobacteriaceae family able to produce polar flagella when grow in liquid medium and lateral flagella when grown in solid or semisolid media. In this study on P. shigelloides 302-73 strain, we found two different gene clusters, one exclusively for the lateral flagella biosynthesis and the other one containing the biosynthetic polar flagell...

To investigate whether mesodermal expansion in the area vasculosa is caused by tension produced by outward migration of cells either in the somatic mesoderm or at the mesodermal edge on an ectodermal substratum, stage 18-20 embryos were transferred to a culture dish. There mesodermal expansion proximal to an arrested edge could be compared with that proximal to a moving edge by measuring the am...

We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 micrograms of chromosomal DNA. In a 1-day protocol, the DNA was isolated, restricted with endonucleases, and fractionated on an agarose gel. Ch...

In order to increase the use of biogas plants in Korea, a farm-scale biogas plant with a working volume of 200 m was constructed at the pig farm in Sung Hwan, Korea as a demonstration project supported by the Korean government. This plant mainly consists of an anaerobic digester processing daily 10 m pig manure and a dual-fuel engine-generator to produce heat and electricity. Digestion takes pl...

a week. On the day of fourth injection, when I gave him 0.1 gramme, suddenly he complained of severe abdominal pain and pressed his hand to his abdomen and after a couple of minutes he became unconscioushis breathing was very hard, pulse was very slow an" almost imperceptible and saliva was coming out of his mouth profusely. I was very much alarmed at these symptoms of the patient. I immediatel...