Artificial insemination (AI) using chilled ram semen has not been adopted in Uruguay yet, probably due to a lack of information about the use of this technology in the country. This study evaluated the fertility rate obtained in Merino ewes inseminated with ram semen stored at 5 °C in a TRIS-based extender in Uruguayan field conditions. The effect of storage period of semen (Experiment 1) and d...

This study was aimed to investigate the effects of omega-3, 6, 9 fatty acids on the characteristics of bovine chilled and frozen-thawed semen. For this purpose, oil containing different levels of omega-3, 6, 9 fatty acids were added to semen extender. To emulsify the oil in semen extender, polyethylene glycol (PEG) was added as a suitable solvent and the solution was finally sonicated. Five pro...

This study was aimed to investigate the effects of omega-3, 6, 9 fatty acids on the characteristics of bovine chilled and frozen-thawed semen. For this purpose, oil containing different levels of omega-3, 6, 9 fatty acids were added to semen extender. To emulsify the oil in semen extender, polyethylene glycol (PEG) was added as a suitable solvent and the solution was finally sonicated. Five pro...

This study was conducted to examine if L-carnitine supplementation in the Tris-egg-yolk extender can improve the motility characteristics, membrane integrity, and in vitro fertilization potential after cryopreservation of ejaculated bovine spermatozoa. It was carried out on January to May 2013 at the University of Wisconsin-Madison. L-carnitine at final concentrations of 0.5, 1, 10, and 30mM we...

Abstract The aim of this study was to determine the effects gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli bucks were used at their breeding season, and ejaculates collected by an electroejaculator. Mixed semen divided into following four groups: control (0 mM), low (L; 1 medium (M; 2 high (H; 4 mM) concentration GA. All groups frozen thawed in a water bath for spermatological...

AIM Sperm membrane cholesterol influences cryodamage during cryopreservation. The present study was carried out to evaluate the effect of varying cholesterol levels in Tris based extenders on the freezability of sexually healthy Malabari buck semen. MATERIALS AND METHODS A total of 48 ejaculates from two adults healthy sexually healthy Malabari bucks were utilized for the study. The collected...

Most strategies for mRNA isolation involve as a first step isolation of total RNA and as a second step selection of poly A + RNA by affinity chromatography on oligo dT cellulose columns. Here we report a novel method which allows isolation of poly A + RNA directly from crude plant tissue without first preparing total RNA or any other purification steps. The method is based upon the addition of ...

Semen preservation and artificial insemination in South American camelids are reviewed giving emphasis to work done in Peru and by the authors. Reports on semen evaluation and the preservation process indicate that semen of alpacas and llamas can be manipulated by making it liquid first. Collagenase appears to be the best enzyme to eliminate viscosity. Tris buffer solution maintains a higher mo...