The objective of this study was to compare fixed-time AI pregnancy rate in Angus crossbred beef cows inseminated with frozen-thawed or fresh-extended semen. Two ejaculates from each of two Angus bulls were collected by artificial vagina and pooled for each bull. The pooled semen from each bull was divided into two aliquots; Aliquot 1 was extended using Caprogen (LIC, Hamilton, New Zealand) to a...

This study's goal was to reveal the effects of antioxidant supplementation on motility, motion characters, morphology, DNA integrity, and fertilizing potential cryopreserved bovine sperm. The ejaculates were collected from three Holstein bulls. At least ten per bull. immediately separated into five aliquots, diluted in Cryobos (Magapor Co. Ltd., Zaragoza, Spain) commercial extender, including 2...

the effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°c was evaluated. semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. semen samples were pooled and divided into 4 parts. each part was diluted with...

The present study was undertaken to assess the effect of trehalose on sperm parameters, enzymatic profiles and biochemical profiles of seminal plasma of mithun. The semen ejaculates were collected from mithun and was split into four groups as group 1: semen without additives (control), group 2 to 4: semen with 50 mM, 75 mM and 100 mM of trehalose, respectively in EYTC extender. These parameters...

Since the conclusion of data collation from previously published work, a further 352 inseminations using frozen-thawed dog semen by endoscopic-assisted transcervical insemination (EIU) have been performed by the author. Insemination was performed on the second day in which crenulation of the anterior vagina was detected in conjunction with a progesterone concentration of >10 ng/ml. All semen sa...

Current Status of AI Use The USA swine breeding herd has been reported to include 3.9 million sows. Approximately 60% of these sows were determined to have been mated by artificial insemination. Each sow received 6.5 doses of semen annually, for a total of 25 million doses utilized in a single year (1999, Boar Semen Conference). Most of the AI is performed with 3 billion sperm in 80 cc of a 5-d...

AIM The present study was designed to evaluate the effect of supplementation of glutamine and replacement of Tris-egg yolk (TE) based buffer with defatted cow milk on the spermatozoa quality after equilibration and thawing. MATERIALS AND METHODS Semen was collected from five Bhadawari bulls biweekly, and a total of 30 ejaculates were taken. The semen ejaculates were pooled and divided into th...

BACKGROUND This study was conducted to evaluate the potential protective effects of omega-3 poly unsaturated fatty acids (Ω-3 PUFAs) on bovine sperm quality in response to cooling and cryopreservation. MATERIALS AND METHODS : In this experimental study included ejaculates from five proven fertile bulls, allocated to the control and the four experimental groups. For group 1, polyethylene glyco...

This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently su...

the aim of this study was to compare the effects of using different levels of royal jelly in semen extender on viatal characteristics of goat sperm after freezing. semen was collected from four adult bucks twice a week and samples pooled together. sperm samples (six repeats) containing royal jelly in four levels (0.5, 1, 1.5 and 2%) had been frozen. after semen samples freezing – were thawed an...