this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

semen analysis, sperm dna fragmentation (sdf) and hypo-osmotic swelling test (host) are usually performed for the evaluation of sperm fertilizing ability. there are some debates over the necessity of sdf and host incorporation in male infertility work-up.semen of 77 men was evaluated by sdf and host through three semen analyses. sperm parameters were arranged into different categories: < 5%, 5-...

The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...

This study compared a number of semen parameters of two separate groups of the common marmoset monkey (Callithrix jacchus) in order to determine the effect of a continuous potentially stressful situation on these parameters, and thus on the monkey's reproductive ability. The semen from 16 adult male marmoset monkeys was collected and analysed to compare semen parameters between a 'normal' (cont...

STUDY QUESTION What is the relationship between body size, physical activity and semen parameters among male partners of couples attempting to become pregnant? SUMMARY ANSWER Overweight and obesity are associated with a higher prevalence of low ejaculate volume, sperm concentration and total sperm count. WHAT IS KNOWN ALREADY Higher BMI is associated with impaired semen parameters, while in...

Semen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to...

We aimed to determine the impact of metabolic syndrome (MetS) on reproductive function in men with secondary infertility, a condition that has received relatively little attention from researchers. Complete demographic, clinical, and laboratory data from 167 consecutive secondary infertile men were analyzed. Health-significant comorbidities were scored with the Charlson Comorbidity Index (CCI; ...

This study was designed to evaluate the relationship between sperm factors and penetration capacity in an in vitro system with immature porcine oocytes. The sperm parameters evaluated in 145 ejaculates were volume, sperm concentration, total cells in the ejaculate, ATP content, morpho-anomalies, percentage of motile sperm cells, forward progressive motility (FPM), acrosome status (NAR), hypo-os...

conclusions these results suggest that serum cholesterol, phospholipids and triacylglycerols have no effect on the levels of cholesterol, phospholipids and triacylglycerols of spermatozoa and seminal plasma. our findings suggest that sperm lipid content is regulated locally within the male reproductive tract. patients and methods cholesterol, phospholipids and triacylglycerols in sperm cells, s...