The interconversions of C1-substituted folates form the core of plant one carbon metabolism. An analytical method for extraction, purification and liquid chromatography−electrospray-ionization mass spectrometry detection of low level, labile tetrahydrofolate monoglutamates from Arabidopsis thaliana was developed in this study. Under gold fluorescent light, folates were extracted by homogenizing...

A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, and phosphoric acid mobile phase and a bonded cyano-amino-type polar phase column has been developed for the rapid, selective, and accurate quantitation of psilocybin in dry mushroom material. A simple one-step procedure is used for the quantitative extraction of psilocybin in under 60 min. The 267:254 nm ...

A new technique for separation and dctermination of ATP, ADP, AMP, NAD', NADP', NADH and NADPH in Lactobacillus plantarum has been developped. It involves acid and basic extraction of nucleotide pool, analysis by reverse-phase high performance liquid chromatography on a 5 p Spherisorb ODS-1 column and UV detection. The method offers advantages in accurate estimations of adenylic and nicotinamid...

A novel light-emitting diode-induced polymerization technology was demonstrated, which could be applied for the on-line construction of an in-column molecularly imprinted solid phase extraction concentrator for capillary electrophoresis. Such a strategy exhibited the advantages of simplicity, zero dead volume, and ease of renewing the concentrator.

Pepticinnamins A, B, C, D, E and F, a family of farnesyl-protein transferase (FPT) inhibitors were isolated from the fermentation broth of Streptomyces sp. OH-4652. These inhibitors were purified from whole broth by extraction with chloroform, followed by silica gel column chromatography, Sephadex LH-20 chromatography and reverse phase HPLC. Among these, pepticinnamin C showed the most potent i...

based on the importance of drops' behavior in liquid-liquid extraction, the maximum sauter mean drop diameter has been investigated and correlated in a counter-current spray extraction column with two chemical systems. spargers were set of nozzles in all experiments. studying the effects of several parameters on drops size, some correlations were estimated by the last available version of softw...

HPLC methods have been developed for the assay of bisacodyl in various pharmaceutical forms. The extraction procedures are simple and the HPLC conditions separate bisacodyl from its degradation products. The chromatography was performed using a Merck LiChrospher RP-select B column, a mobile phase of 55% acetonitrile/45% 0.05 M KH2PO4 and detection by UV at 214 nm.

A reversed-phase salt assisted liquid-liquid extraction (RP-SALLE) method was developed for the preconcentration and determination of trace crocin in herbal medicines by high-performance liquid chromatography (HPLC). In developed method, the roles of the aqueous and organic phases are inverted so that the analyte is extracted from the organic into the aqueous phase after salt addition and phase...