The KNUC25 strain isolated from over-fermented whole Chinese cabbage kimchi was examined for its physiological characteristics using API 50 CHL system assay and identified as Lactobacillus paraplantarum by analysis of whole-cell protein SDS-PAGE pattern assay and similarity of 16S rDNA sequence. L. paraplantarum KNUC25 had a broad antimicrobial activity spectrum from Gram positive to Gram negat...

Nonlinear filtering can solve very complex problems, but typically involve very time consuming calculations. Here we show that for filters that are constructed as a RBF network with Gaussian basis functions, a decomposition into linear filters exists, which can be computed efficiently in the frequency domain, yielding dramatic improvement in speed. We present an application of this idea to imag...

We have taken random image noise into consideration in the contrast-to-gradient (CG) method for the evaluation of image resolution R in scanning electron microscopy (SEM). When looking at the local fine pattern in the SEM micrograph containing much random noise, viewers gradually expand the region of interest (ROI) to improve the signal-to-noise ratio (SNR) and to recognize the pattern. We empl...

Accurate determination of micrograph focuses is essential for averaging multiple images to reach high-resolution 3-D reconstructions in electron cryo-microscopy (cryo-EM). Current methods use iterative fitting of focus-dependent simulated power spectra to the power spectra of experimental images, with the fitting performed independently for different images. Here we have developed a novel graph...

The aim of this study was to investigate the activity of diosgenin against Naegleria fowleri trophozoites at the cellular and molecular levels. Diosgenin (100 μg/ml; 241.2 μM) had a 100% inhibitory effect on N. fowleri trophozoites (5 x 10(5) cell/ml). Scanning electron micrograph revealed diosgenin decreased the number of sucker-like apparatuses and food cup formation among N. fowleri trophozo...

Three-dimensional reconstruction of large macromolecules like viruses at resolutions below 10 A requires a large set of projection images. Several automatic and semi-automatic particle detection algorithms have been developed along the years. Here we present a general technique designed to automatically identify the projection images of particles. The method is based on Markov random field mode...

2014 Examples are shown that illustrate rapid changes that can take place while a thin foil specimen is under observation in a high-voltage microscope. These unusually dramatic examples emphasize the fact that for correct characterization of structural details of any crystalline material by lattice imaging microscopy particularly at surfaces, interfaces, and other defects, it is essential never...

Size-selected TiN nanoclusters in the range of 4 to 20 nm have been produced by an ionized cluster beam, which combines a glow-discharge sputtering with an inert gas condensation technique. With this method, by controlling the experimental conditions, it was possible to produce nanoparticles with a high control in size. The size distribution of TiN nanoparticles was determined before deposition...

introduction: this study was designed to investigate the characters of the human endometrial epithelial cells when cultured on either plastic or ecm (extracellular matrix) gel surface and to study ultrastructural features of cultured cells in comparison to endometrial tissue. material and methods: human endometrial tissue was obtained from patients who had undergone hysterectomy for uterine myo...

Abstract The SiO2-LiBr hybrid porous materials were prepared by the sol-gel method. This process was obtained by the hydrolysis and condensation Tetraethyl orthosilicate (TEOS) with replacement of ethanol from alcogel by the drying at ambient temperature to obtain xerogel structure. The alcogel samples were synthesized from TEOS, EtOH, H2O, HCl, NH4OH and LiBr. The total molar ratio of the comp...