Scaffold proteins regulate cell signalling by promoting the proximity of putative interaction partners. Although they are frequently applied in cellular settings, fundamental understanding of them in terms of, amongst other factors, quantitative parameters has been lagging behind. Here we present a scaffold protein platform that is based on the native 14-3-3 dimeric protein and is controllable ...

Assembly of certain classes of bacterial and animal viruses requires the transient presence of molecules known as scaffolding proteins, which are essential for the assembly of the precursor procapsid. To assemble a procapsid of the proper size, each viral coat subunit must adopt the correct quasiequivalent conformation from several possible choices, depending upon the T number of the capsid. In...

Method: Studies were made on the treatment of lumbar herniated disc in CINAHIL, PubMed, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL) and Cochrane Database of Systematic Reviews(CDSR). This paper provides methods for restoration of flexion in the bone. A biomaterial scaffold made of poly-β-hydroxybutyrate (PHB) consisting of extracellular matrix (ECM) proteins which is synthe...

The human Usher syndrome (USH) is the most frequent cause of combined deafblindness in man. It is clinically and genetically heterogeneous and at least twelve chromosomal loci are assigned to three clinical USH types. USH1, USH2 and USH3 differ in the severity of the symptoms: hearing loss, balance problems and retinal degeneration namely retinitis pigmentosa (RP), as well as in the progression...

traumatic brain injury (tbi) results from a sudden and external physical impact to the head, and often leads to motor and cognitive impairment. using tissue engineering strategies, we aimed to review aspects of extra cellular matrix (ecm) based scaffold that could be delivered to the injured brain. the ecm is produced intracellularly and secreted to form a dense network of proteins and glycans,...

Abstract Cellular liquid–liquid phase separation is a physiologically inevitable phenomenon in molecularly crowded environments inside cells and serves to compartmentalize biomolecules facilitate several functions, forming cytoplasmic nuclear RNA granules. Abnormalities the process granules are implicated onset of neurodegenerative diseases; initial liquid-like phase-separated droplets containi...

HER2-positive breast cancer occurs in 15–20% of patients and is associated primarily with a poor prognosis the disease need for highly specific targeted therapy. Despite clinical importance determining HER2/neu, traditional diagnostic methods have their disadvantages require study new additional research techniques. The information presented this review makes it possible to consider current tre...

Members of the intersectin (ITSN) family of scaffold proteins consist of multiple modular domains, each with distinct ligand preferences. Although ITSNs were initially implicated in the regulation of endocytosis, subsequent studies have revealed a more complex role for these scaffold proteins in regulation of additional biochemical pathways. In this study, we performed a high throughput yeast t...

We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase...

The ability to design and construct structures with atomic level precision is one of the key goals of nanotechnology. Proteins offer an attractive target for atomic design because they can be synthesized chemically or biologically and can self-assemble. However, the generalized protein folding and design problem is unsolved. One approach to simplifying the problem is to use a repetitive protein...