Specific transcription in late G1, mediated by the transcription factors SBF (Swi4p-Swi6p) and MBF (Mbp1p-Swi6p), is crucial for cell cycle progression in budding yeast. In order to better understand the G1/S transition, we initiated a search for conditional mutations synthetic lethal with swi4delta. One of the isolated mutants, rsf8swi4delta, showed a growth defect due to cell lysis. rsf8 is a...

The combination of the high mechanical strength of metals with the osteoconductive properties of calcium phosphates make hydroxyapatite coatings on titanium implants widely used in orthopedic surgery. However, the most popular coating method, plasma spraying, exhibits some important drawbacks: the inability to cover porous implants and to incorporate biologically active agents, delamination, an...

In the yeast Saccharomyces cerevisiae, SBF (Swi4-Swi6 cell cycle box binding factor) and MBF (MluI binding factor) are the major transcription factors regulating the START of the cell cycle, a time just before DNA replication, bud growth initiation, and spindle pole body (SPB) duplication. These two factors bind to the promoters of 235 genes, but bind less than a quarter of the promoters upstre...

Apatite layers were grown on the surface of newly developed starch/polycaprolactone (SPCL)-based scaffolds by a 3D plotting technology. To produce the biomimetic coatings, a sodium silicate gel was used as nucleating agent, followed by immersion in a simulated body fluid (SBF) solution. After growing a stable apatite layer for 7 days, the scaffolds were placed in SBF under static, agitated (80 ...

One method for making analogies is to access and instantiate abstract domain principles, and one method for acquiring knowledge of abstract principles is to discover them from experience. We view generalization over experiences in the absence of any prior knowledge of the target principle as the task of hypothesis formation, a subtask of discovery. Also, we view the use of the hypothesized prin...

PURPOSE The goal of this study was to compare the in vitro bioactivity in simulated body fluid (SBF) of commercially available dental implants. MATERIALS AND METHODS Bioactivity, according to ISO 23317, of commercially available dental implants with various surface modifications (BIO-surface, SLA, SLActive, TiUnite, and OsseoSpeed) was tested in SBF for 1 and 3 weeks. Surface characterization...

Retinal degenerative conditions can vary in their clinical features and often present with subtle phenotypic features before the onset of clinically overt disease. To capture these isolated events that precipitate disease, large representative areas of the retina must be imaged at high resolution. Compared to light microscopic methods, traditional electron microscopy can provide images at suffi...

OBJECTIVES Ti, which is biocompatible and resistant to corrosion, is widely used for dental implants, particularly in patients allergic to other materials. However, numerous studies have reported on Ti allergy and the in vitro corrosion of Ti. This study investigated the conditions that promote the elution of Ti ions from Ti implants. METHODS Specimens of commercially pure Ti, pure nickel, a ...

Budding yeast cells irreversibly commit to a new division cycle at a regulatory transition called Start. This essential decision-making step involves the activation of the SBF/MBF transcription factors. SBF/MBF promote expression of the G1 cyclins encoded by CLN1 and CLN2. Cln1,2 can activate their own expression by inactivating the Whi5 repressor of SBF/MBF. The resulting transcriptional posit...