A cytomegalovirus (CMV)-specific sandwich hybridization test was constructed by using two adjacent BamHI DNA fragments of CMV DNA as reagents. The fragments were cloned into two different vectors. One of the recombinants was attached to the filter, and the other was the labeled probe. When present in the sample, CMV DNA mediated labeling of the filter by hybridizing to both the filter-bound DNA...

conclusions the current study revealed that a significant percentage of sandwich generation females have chronic diseases. results a statistically significant difference was found between the two groups in terms of the presence of chronic diseases (p = 0.001). there was a significant correlation between chronic diseases and number of children (p = 0.03), person receiving care (p = 0.004), educa...

in this paper‎, ‎we prove a multiplicity result for some biharmonic elliptic equation of kirchhoff type and involving nonlinearities with critical exponential growth at infinity‎. ‎using some variational arguments and exploiting the symmetries of the problem‎, ‎we establish a multiplicity result giving two nontrivial solutions‎.

Herein paper compares the analytical model with the FEM based numerical model of the axisymmetric bending of circular sandwich plates. Also, the paper describes equations of the circular symmetrical sandwich plates bending with isotropic face sheets and the nonlinear elastic core material. The method of constructing an analytical solution of nonlinear differential equations has been described. ...

In the present article, we define a new family for holomorphic functions (so-called Bazilevic-Sakaguchi type functions) and determinate strong differential subordination superordination results these by investigating certain suitable classes of admissible functions. These are applied to obtain sandwich results.

BACKGROUND Women are disproportionately likely to assist aging family members; approximately 53 million in the United States are involved with the health care of aging parents, in-laws, or other relatives. The busy schedules of "sandwich generation" women who care for older relatives require accessible and flexible health education, including Web-based approaches. OBJECTIVE This paper describ...

Sandwich construction is widely accepted as a method of construction especially in the aircraft industry. It is a type of stressed skin construction formed by bonding two thin faces to a thick core, the faces resist all of the applied edge loads and provide all or nearly all of the required rigidities, the core spaces the faces to increase cross section moment of inertia about common neutral ax...

This highly sensitive immunoenzymometnc assay method involves monoclonalantibodies, a common-capturemicrosphere, and a rapid, membrane-filtration separation step. The common-capture solid phase is monoclonal anti-fluorescain antibody covalently attached to 6.5-sm-diameter latex particles. In sandwich-type assays for large-molecule analytes, the capture antibody is conjugated with fluorescein Is...

Steered molecular dynamics (SMD), a computer simulation method for studying force-induced reactions in biopolymers, has been applied to investigate the response of protein domains to stretching apart of their terminal ends. The simulations mimic atomic force microscopy and optical tweezer experiments, but proceed on much shorter time scales. The simulations on different domains for 0.6 nanoseco...

The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to the target. To bridge this gap, we invented Megaprimer Shuffling for Tandem Affinity Reagents (MegaSTAR) to ...