various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several hyoscyamus species (i.e. h. pusillus, h. arachnoideus, annual and biennial h. niger from two distinct geographical regions) were cultured on ms media with various growth regulator combinations, to assess the ability of flower production. statistical assessments showed...

mycoplasma agalactia was cultured in pplo broth medium supplemented with sterilized normal horse serum in fermentor. cultivation process continued at 37 ºc under aerobic conditions and growth was terminated after 26 hours cultured period with 2.2×109 mycoplasma per ml. samples were taken at different stages of growth. culture was inactivated with 0.04 %v/v formaldehyde and the vaccine was adjuv...

The Phomopsis is a genus of imperfect plant pathogenic fungus whose hosts comprise several species in different regions of the world, such as grapes, soybean, acacia, hollyhock, velvetleaf, and several other plants. In this study, samples collected from hollyhock, velvetleaf, purple bauhinia, and acacia plants suspected to be infected with Phomopsis fungi. Samples were culture...

Respiratory samples from cystic fibrosis outpatients were cultured on Sabouraud's dextrose agar (SABD) containing antibiotics, Mycosel, and Scedosporium-selective medium (SceSel+). Thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a Scedosporium sp., most frequently Scedosporium aurantiacum (17/218). Scedosporium was recovered on SceSel+, Mycosel, and SABD from 90.6%, 50.0...

Six strain of Trichoderma (T. viride, T. harzianum, T. reesei, T. longibrachiatum, T. koningii, T. virens) were isolated and used for extracellular enzyme production. Species of Trichoderma are collected from the surrounding soil of suger beet root fields in East Iran, and held in vitro cultured PDA. TCM and TFM are used for the growth of spores and induceing the production of Cellulase. Finall...

We have developed a new, effective procedure for detecting Vibrio parahaemolyticus in seafoods using enrichment and plating onto a chromogenic agar medium. Samples were cultured in salt Trypticase soy broth, which is a nonselective medium, and then a portion of the culture was cultured with salt polymyxin broth, which is a selective medium for V. parahaemolyticus. This two-step enrichment was m...

Introduction: Marine actinomycetes have a great potential to produce unique bioactive compounds due to their special adaptation in the harsh ocean environment. The current study aimed to isolate anti-cancer compounds producing actinomycetes from sediments of Harra forests of the Persian Gulf and investigate their potential as anti-breast cancer metabolites. Methods: In the current study, 40 se...