Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...

For a modern agricultural, the search for sustainable practices to increase productivity is fundamental. Steel slag have been studied their potential use in agriculture. These substances present great possibility of agricultural applications since they are rich nutrients, which enhance plant uptake. In this regard, effect steel based-fertilizer was investigated greenhouse durum wheat cultivatio...

Experiments were performed to determine if protein A influenced the association of bacteriophages with Staphylococcus aureus. Bacteriophage adsorption was compared in a S. aureus strain rich in protein A and mutants of this strain with very little protein A, in a strain with little protein A, and in mutants of this strain with increased protein A. In addition, the effect of growth in mannitol-s...

Brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae that evolved complex multicellularity. is dominant seaweed in temperate regions, abundant mostly the intertidal zones, an environment with high levels abiotic stresses. Previous transcriptomic analysis revealed several genes consistently induced by various stresses; one these Esi0017_0056, which encodes protein unknown fun...

tobacco is a salt-sensitive glycophyte crop species. in this work effect of silicone (si) supplementation (1 mm as na2sio3) was studied in nicotiana rustica l. cv. basmas grown hydroponically in growth chamber under control, low (25 mm) and high (75 mm) nacl concentration for two weeks. dry matter production of leaves was depressed by salinity level as low as 25 mm and higher salt concentration...

Onion (Allium cepa L.) is a biennial crop of high commercial value in Pakistan. considered as salt sensitive plant species. The present investigation was carried out to investigate the effect salinity on onion and its alleviation through exogenously applied gibberellic acid (GA3; 100 mg L-1). Foliar application GA3 (100 L-1) seedlings grown under three levels (0, 2 or 4 dS m-1) after 45 days so...

Salt-affected soils are related to salinity (high content of soluble salts) and/or sodicity (excess sodium), which major leading causes agricultural land degradation. This study aimed evaluate the performances three machine learning (ML) algorithms in predicting soil exchangeable sodium percentage (ESP), electrical conductivity (ECe), and salt-affected classes, from salt ions. The assessed ML m...

High salinity seriously affects the production of chrysanthemum, so improving the salt tolerance of chrysanthemum becomes the focus and purpose of our research. The WRKY transcription factor (TF) family is highly associated with a number of processes of abiotic stress responses. We isolated DgWRKY4 from Dendranthema grandiflorum, and a protein encoded by this new gene contains two highly conser...

The Saccharomyces cerevisiae respiratory supercomplex factor 2 (Rcf2) is a 224-residue protein located in the mitochondrial inner membrane where it involved formation of supercomplexes composed cytochrome bc1 and c oxidase. We previously demonstrated that Rcf2 forms dimer dodecylphosphocholine micelles, here we report solution NMR structure this dimer. Each monomer has two soluble α helices fiv...