Most melanoma cells are characterized by the V600E mutation in B-Raf kinase. This mutation leads to increased expression of interleukin (CXCL8), which plays a key role in cell growth and angiogenesis. Thus CXCL8 appears to be an interesting therapeutic target. Hence, we performed vaccination of mice with GST-CXCL8, which results in a reduced incidence of syngenic B16 melanoma cell xenograft tum...

Synthetic capped RNA transcripts injected into fertilized eggs of Xenopus laevis have a half-life of 3-4 h. Addition of a long (approximately 200 nucleotide) poly(A) tail increases the half-life to 6-8 h which approaches the half-life of natural polyadenylated globin RNA injected into embryos. Since exonucleolytic action alone could account for the degradation of RNA, we tested whether circular...

In this paper we present an Whiteheadean style point-free theory of space and time. Here ”point-free” means that neither space points, nor time moments are assumed as primitives. The algebraic formulation of the theory, called dynamic contact algebra (DCA), is a Boolean algebra whose elements symbolize dynamic regions changing in time. It has three spatio-temporal relations between dynamic regi...

Studies are presented on the ability of low-molecular-weight nuclear DNA fractions to hybridize to higher-molecular-weight nuclear DNAs taken from different stages of early developing sea-urchin embryos, Strongylocentrotus purpuratus. Using preparative DNA-DNA hybridization, a fraction of 60-s mid-blastula DNA was isolated for its ability to anneal to 10-s morula DNA. Approximately 80 regions o...

objective(s):the present study investigated the effect of long-term mild cerebral hypoperfusion induced by permanent unilateral (right) common carotid artery occlusion (uco) on the dorsal hippocampal neurons in rats. materials and methods:sixty four male sprague-dawley rats aged 4 months were divided into two groups of sham and uco. these two groups were further divided into 4 sets of histopath...

background: cone-beam computed tomography (cbct) is a new imaging technology that has been widely used in implantology, oral and maxillofacial surgery and orthodontics. this method provides 3-d images that are composed of voxel, which is the smallest image unit, and determines image resolution. smaller voxel is associated with the higher resolution and also greater radiation exposure. this stud...

Mercury is a potent toxicant of concern to both the general public and occupationally exposed workers (e.g., dentists). Recent studies suggest that several genes mediating the toxicokinetics of mercury are polymorphic in humans and may influence inter-individual variability in mercury accumulation. This work hypothesizes that polymorphisms in key glutathione synthesizing enzyme, glutathione S-t...

NifA protein activates transcription of nitrogen fixation operons by the alternative sigma 54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS) located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST)-NifA fusion pr...

BACKGROUND Glutathione S-transferase P1 (GSTP1) plays a role in a spectrum of respiratory diseases; however, the effects of sequence variation across the entire locus in asthma pathogenesis have yet to be determined. OBJECTIVES This study was designed to investigate whether sequence variations in the GSTP1 coding and promoter regions are associated with asthma and wheezing outcomes and to det...

Hypermethylation of the 5' promoter region of the glutathione S-transferase pi gene (GSTP1) occurs at a very high frequency in prostate adenocarcinoma. We compared the results of blinded histologic review of sextant biopsy samples from 72 excised prostates with those obtained using a quantitative methylation-specific polymerase chain reaction assay (QMSP) for GSTP1. Formal surgical pathologic r...