In the filamentous fungus Podospora anserina, two phenomena are associated with polymorphism at the het-s locus, vegetative incompatibility and ascospore abortion. Two het-s alleles occur naturally, het-s and het-S. The het-s encoded protein is a prion propagating as a self-perpetuating amyloid aggregate. When prion-infected [Het-s] hyphae fuse with [Het-S] hyphae, the resulting heterokaryotic ...

The molecular basis for the genetic polymorphism of thiopurine S -methyltransferase (TPMT) has been estab-lished for Caucasians, but it remains to be elucidated in African populations. In the current study, we determined TPMT genotypes in a population of 248 African-Americans and compared it with allele frequencies in 282 Caucasian Americans. TPMT genotype was determined in all individuals with...

the innate immune system recognizes the presence of bacterial products through the expression of a family of membrane receptors known as toll-like receptors (tlrs). polymorphisms in tlrs have been shown to be associated with increased susceptibility to diseases such as inflammatory bowel disease. the aim of this study was to determine whether there was a correlation between polymorphisms of tlr...

dear editor analysis of the phenylalanine hydroxylase (pah mckusick 261600) gene in different populations has revealed more than 320 different mutations associated with phenylketonuria (pku). one of these mutations, ivs10nt546, results in severe pah deficiency due to defective mrna splicing. it accounts for about 40 percent of all mutant alleles in turkish and between 10 to 20 percent of all mu...

BACKGROUND Selective amplification of minority alleles is often necessary to detect cancer mutations in clinical samples. METHODS Minor-allele enrichment and detection were performed with snapback primers in the presence of a saturating DNA dye within a closed tube. A 5' tail of nucleotides on 1 PCR primer hybridizes to the variable locus of its extension product to produce a hairpin that sel...

Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific ...

The present research aims to study the association and allelic diversity of linked microsatellite markers to grain quality QTLs of 84 exotic rice genotypes. To this end, 9 microsatellite markers (RM540, RM539, RM587, RM527, RM216, RM467, RM3188, RM246, RM5461) were used in which a total of 61 alleles were identified with a mean of 6 alleles per locus. The polymorphism information content (PIC) ...