The aim of this study was to investigate the variations of vitamin B12 and homocysteine in blood of dairy cows during subacute ruminal acidosis (SARA). On 228 subjects ruminal liquid was collected through rumenocentesis technique and rumen pH was immediately measured by a portable pH-meter. On the basis of pH values all cows were classified (bovine class) in Group A (animals with rumen pH>5.7),...

Biogas productions of cattle manure using rumen fluid inoculums were determined using batch anaerobic digesters at mesophilic temperatures (room and 38.5 C). The aim of this paper was to analyze the influence of rumen fluid contents on biogas yield from cattle manure using fluid rumen inoculums. A series of laboratory experiments using 400 ml biodigester were performed in batch operation mode. ...

The goal of the Hungate1000 project is to generate a reference set of rumen microbial genome sequences. Toward this goal we have carried out a meta-analysis using information from culture collections, scientific literature, and the NCBI and RDP databases and linked this with a comparative study of several rumen 16S rRNA gene-based surveys. In this way we have attempted to capture a snapshot of ...

Cultures of Streptococcus bovis and mixed populations of rumen bacteria were used to investigate the concentration of ATP and rumen bacterial numbers at various stages of growth. ATP, extracted with Tris buffer, was analyzed using the firefly luciferin-luciferase bioluminescent reaction. ATP concentrations of S. bovis and mixed cultures of rumen bacteria significantly correlated with live cell ...

The manufacturing processes of royal demolition explosive (RDX), or hexahydro-1,3,5-trinitro-1,3,5-triazine, have resulted in serious water contamination. As a potential carcinogen, RDX can cause a broad range of harmful effects to humans and animals. The ovine rumen is capable of rapid degradation of nitroaromatic compounds, including RDX. While ruminal RDX-degrading bacteria have been identif...

Vaccination against rumen methanogens offers a practical approach to reduce methane emissions in livestock, particularly ruminants grazing on pasture. Although successful vaccination strategies have been reported for reducing the activity of the rumen-dwelling organism Streptococcus bovis in sheep and S. bovis and Lactobacillus spp. in cattle, earlier approaches using vaccines based on whole me...

Ruman degradation is crucial in the supply of dietary nutrients to meet the nutrient demands of the anaerobic microbes and body tissues of ruminant animals. Therefore, it is essential to study the dynamics of rumen degradation of various feeds before their potential use to formulate nutritious diets for ruminant animals. Amongst many methods that have been used in the past, the in sacco method ...

Feeding dairy cows diets high in easily degradable carbohydrates increases the incidence of rumen and systemic metabolic disorders; however, the triggering factor is not well understood. In this study, dairy cows were fed 4 different amounts of barley grain-based concentrate at 15, 30, 45, and 60% (dry matter basis) of a total mixed ration to determine whether alterations in the rumen environme...

Numerous studies have been made of the quantitative aspects of the production of volatile fatty acids (VFA) in the rumen, and have been reviewed by Rook (1964), Warner (1964) and Annison (1965). Interest in rumen VFA production has recently been intensified because of the findings correlating body-weight increases with the concentration of rumen VFA in lambs on various pastures (Johns, Ulyatt &...

limited data exist about the production of extruded linseed as a feed ingredient in farm animal nutrition. the objective of this study was to produce and evaluate extruded linseed mixed with alfalfa hay, pistachio by-products, and sugar beet pulp or corn grain in different proportions of linseed: alfalfa hay: pistachio by-products at ratios of 70:15:15, 70:20:10, 70:10:20, and 80:10:10 for qual...