نتایج جستجو برای: rt qpcr

تعداد نتایج: 69636  

Journal: :Journal of clinical microbiology 2017
Felicia Roy Lillian Mendoza Joanne Hiebert Rebecca J McNall Bettina Bankamp Sarah Connolly Amy Lüdde Nicole Friedrich Annette Mankertz Paul A Rota Alberto Severini

During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A measles virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles...

Journal: :International Journal of Infectious Diseases 2020

Journal: :The International Arabic Journal of Antimicrobial Agents 2021

Background: The study aims to evaluate the antibacterial activity of Trigona honey against S. pneumonia. Methods: effect on pneumonia investigated using agar well diffusion, MIC, MBC, biofilm formation and RT-qPCR.
 Results: samples showed larger zones inhibition pneumonia, 22.2±0.4 at 100% concentration. possessed lowest MIC50 MIC90 pneumoniae, 25%, 30%, 12.5% 25% (w/v) respectively. perm...

Journal: : 2022


 Many differentially expressed genes associated with feather colour in quail embryos can be screened using RNA-seq technology. The aim of this study was to verify the accuracy RT-qPCR. transcriptome embryonic skin tissues quails were sequenced by Illumina HiSeq TM2000 sequencing platform. As a result, 2512 found. Among them, 950 up-regulated white-feather embryos, and 1562 down-regulated....

Journal: :Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2013
Richard J Whitley Charles A Boucher Bruno Lina Jonathan S Nguyen-Van-Tam Albert Osterhaus Martin Schutten Arnold S Monto

BACKGROUND Following emergence of naturally occurring oseltamivir-resistant influenza A(H1N1) viruses, a global observational investigation, the Influenza Resistance Information Study (IRIS; NCT00884117), was initiated in 2008 to study neuraminidase inhibitor (NAI) resistance and clinical outcome. METHODS Patients with influenza-like illness and/or positive rapid test results agreed to swabs ...

2010
Elena A Takano Thomas Mikeska Alexander Dobrovic David J Byrne Stephen B Fox

BACKGROUND RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies. Most of the current approaches for RNA quality assessment are not suitable for FFPE derived RNA. RESULTS We have developed a single-tube multiplex endpoint RT-PCR assay specifically designed to evaluate RNA extracted from FFPE...

2017
Marcelo Henrique Santos Paiva Duschinka Ribeiro Duarte Guedes Walter Soares Leal Constância Flávia Junqueira Ayres

Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).

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