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Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...

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simultaneous detection of arabis mosaic virus, cherry leafroll virus and cucumber mosaic virus with coamplification of plant mrna as internal control for olive certification programs

Journal: :iranian journal of virology 0

m naderpour seed and plant certification research institute (spcri), p. o. box 31535-1516, karaj, iran r shahbazi seed and plant certification research institute (spcri), p. o. box 31535-1516, karaj, iran l sadeghi seed and plant certification research institute (spcri), p. o. box 31535-1516, karaj, iran h maddah-arefi seed and plant certification research institute (spcri), p. o. box 31535-1516, karaj, iran

background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

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