نتایج جستجو برای: rrna gene

تعداد نتایج: 1153532  

Journal: :Journal of medical microbiology 2008
Andreas Edberg Margaretha Jurstrand Eva Johansson Elisabeth Wikander Anna Höög Thomas Ahlqvist Lars Falk Jørgen Skov Jensen Hans Fredlund

The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time Mycoplasma genitalium adhesin protein (MgPa) gene PCR as detection methods for M. genitalium infection. The study also determined the prevalence of M. genitalium in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First ...

2011
Mohammed Siraj Ud Din Magray Anup Kumar Anil Kumar Rawat Shipra Srivastava

A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia...

Journal: :Journal of clinical microbiology 2013
Marion Walcher Rhonda Skvoretz Megan Montgomery-Fullerton Vivian Jonas Steve Brentano

An apparently rare Neisseria meningitidis isolate containing one copy of a Neisseria gonorrhoeae 16S rRNA gene is described herein. This isolate was identified as N. meningitidis by biochemical identification methods but generated a positive signal with Gen-Probe Aptima assays for the detection of Neisseria gonorrhoeae. Direct 16S rRNA gene sequencing of the purified isolate revealed mixed base...

Journal: :Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing 2011
Neethu Shah Haixu Tang Thomas G. Doak Yuzhen Ye

16S rRNA gene sequencing has been widely used for probing the species structure of a variety of environmental bacterial communities. Alternatively, 16S rRNA gene fragments can be retrieved from shotgun metagenomic sequences (metagenomes) and used for species profiling. Both approaches have their limitations-16S rRNA sequencing may be biased because of unequal amplification of species' 16S rRNA ...

2016
James E. McDonald Niels Larsen Andrea Pennington John Connolly Corrin Wallis David J. Rooks Neil Hall Alan J. McCarthy Heather E. Allison

PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes. However, 'universal' SSU rRNA gene PCR primer sets are likely to miss much of the diversity therein. We sequenced a library comprising purified and reverse-transcribed SSU rRNA (RT-SSU rRNA) molecules from...

Journal: :Veterinary parasitology 2009
Raksha Bhoora Linda Franssen Marinda C Oosthuizen Alan J Guthrie Erich Zweygarth Barend L Penzhorn Frans Jongejan Nicola E Collins

A molecular epidemiological survey of the protozoal parasites that cause equine piroplasmosis was conducted using samples collected from horses and zebra from different geographical locations in South Africa. A total of 488 samples were tested for the presence of Theileria equi and/or Babesia caballi using the reverse line blot hybridization assay. Ten percent of the samples hybridized to the T...

Journal: :Current opinion in cell biology 2010
Sarah Tucker Alexa Vitins Craig S Pikaard

Nucleolar dominance is an epigenetic phenomenon that occurs in genetic hybrids and describes the expression of 45S rRNA genes inherited from one progenitor due to the silencing of the other progenitor's rRNA genes. Nucleolar dominance is a manifestation of rRNA gene dosage control, which also occurs in non-hybrids, regulating the number of active rRNA genes according to the cellular demand for ...

Journal: :Microbiological reviews 1985
B S Vold

INTRODUCTION................................................ 71 ORGANIZATION OF tRNA GENE REGIONS ................................................ o 71 Mapping of tRNA and rRNA Gene Clusters ................................................ 71 Large Clusters of tRNA genes-Evolutionary Considerations ................................................ 73 SEQUENCE ANALYSIS OF tRNA STRUCTURAL GENES .....

Journal: :Nucleic acids research 2001
Joel A. Klappenbach Paul Saxman James R. Cole Thomas M. Schmidt

The Ribosomal RNA Operon Copy Number Database (rrndb) is an Internet-accessible database containing annotated information on rRNA operon copy number among prokaryotes. Gene redundancy is uncommon in prokaryotic genomes, yet the rRNA genes can vary from one to as many as 15 copies. Despite the widespread use of 16S rRNA gene sequences for identification of prokaryotes, information on the number ...

Journal: :Veterinary parasitology 2004
Enara Aguirre Angel Sainz Susana Dunner Inmaculada Amusategui Lissette López Fernando Rodríguez-Franco Inés Luaces Oscar Cortés Miguel A Tesouro

This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the ...

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