Rosehip is one of the richest sources vitamin C, and jam made from it tastes delicious. In this work, content food colors E160a (lycopene) E160d (?-carotene) was determined, as well antioxidant capacity these products. By applying UV-Vis spectrophotometric method using Lambert-Beer law, a system two linear equations with unknowns set up, which used to determine concentrations for E160d. The lyc...

"Bead beating" is commonly used to release DNA from cells for genomic studies but it was used here to prepare suspensions of plant nuclei for measurement of DNA amounts by flow cytometry. Plant material was placed in 2-ml screw-capped tubes containing beads of zirconia/silica (2.5 mm diameter) or glass (2.5 or 1.0 mm diameter) and 1 ml of lysis buffer. The tubes were mechanically shaken with an...

G.A. Gellert1; V. Hill2; K. Bruner1; G. Maciaz1; L. Saucedo1; L. Catzoela1; R. Ramirez1; W.J. Jacobs1; P. Nguyen3; L. Patel4; S.L. Webster1 1CHRISTUS Health, Health Informatics, San Antonio, TX, United States; 2Baylor College of Medicine, Pediatrics, San Antonio, TX, United States; 3CHRISTUS Santa Rosa Westover Hills Hospital, San Antonio, TX, United States; 4CHRISTUS Santa Rosa Medical Center,...

It was during the 1950's that the development of flow cytometry started, technology that allow to measure physiochemical characteristics of cells or suspended particles in fluid. This technology uses monoclonal antibodies labeled by fluorochromes as investigation tool in several analysis and needs isotype controls to define the negative region (background). These controls are constituted by imm...