The interaction of Methylene Blue (MB) with chondroitin-4-sulfate (CHS) has been investigated using spectroscopic techniques, including UV-Vis absorption, Rayleigh resonance scattering (RRS), and circular dichroism (CD). The addition of CHS caused a decrease in the absorbance of MB at 664 nm with a new absorption band appearing at 570 nm, enhanced RRS at 314 nm and 560 nm, and also resulted in ...

A simple, novel and sensitive method was developed to determine ethion insecticide in water samples. This method was based on the interaction of ethion with silver nanoparticles (AgNPs) and quenching of the resonance Rayleigh scattering (RRS) intensity. The change in RRS intensity (ΔIRRS) was linearly correlated to the concentration of ethion over the range of 10.0-900.0 µg L(-1). Ethion can be...

In a diluted H2SO4 solution, Hg(II) reacts with halide anions X- (including Cl-, Br- and I-) to form anionic complexes [HgX4]2- that can further react with berberine to form ion-association complexes of [Ber]2[HgX4]. As a result, the absorption spectra change, their maximum absorption wavelengths are at 230 nm for [Ber]2[HgCl4], 278 nm for [Ber]2[HgBr4] and 300 nm for [Ber]2[HgI4]. However, amo...

Inter-sectional hybrids were successfully obtained by the reciprocal crosses between 11 cultivars (including 6 diploids and 5 tetraploids) of Begonia semperflorens (SS & SSSS genomes) and B. 'Orange Rubra' (RR genome) with the aid of in vitro culture of mature or immature seeds on MS medium containing 0.1 mg l(-1) α-naphthylacetic acid, 0.1 mg l(-1) 6-benzyladenine, 10 mg l(-1) gibberellic acid...

The structure and catalytic properties of the enzyme (E) chlorocatechol dioxygenase (CCD) adsorbed on a citrate-reduced silver colloid are analyzed by surface-enhanced resonance Raman spectroscopy (SERRS). This is the first SERRS study of a non-heme metalloenzyme. It is demonstrated that the native conformation of CCD is retained in the adsorbed state by comparison of resonance Raman scattering...

The white dwarf (i.e., the primary) in the AE Aqr binary star system is observed to spin down at a steady time rate Ṗnow = 5.64 × 10−14 s s−1, while at the same time its UV (LUV ) and X-ray (LX) accretion luminosities seem to remain almost unchanged. This is a contradiction, however, since the classically estimated spin-down power due to decrease of rotational kinetic energy T , Ṫ ∼ −1034 erg s...

Six cycles of reciprocal recurrent selection (RRS) between Line 8 (Duroc) and Line 9 (Beltsville No. 1) were completed at the Fort Reno Livestock Research Center, El Reno, Oklahoma. A randomly mated control line was maintained. Each cycle of selection required three seasons. Selection of purebred pigs (born in the second season) was based on the mean 21-d litter weight of their maternal and pat...

BACKGROUND Necdin, a MAGE family protein expressed primarily in the nervous system, has been shown to interact with both nuclear and cytoplasmic proteins, but the mechanism of its nucleocytoplasmic transport are unknown. METHODOLOGY/PRINCIPAL FINDINGS We carried out a large-scale interaction screen using necdin as a bait in the yeast RRS system, and found a wide range of potential interactors...

The Sleeping Beauty (SB), piggyBac (PB) and Tol2 transposons are promising instruments for genome engineering. Integration site profiling of SB, PB and Tol2 in human cells showed that PB and Tol2 insertions were enriched in genes, whereas SB insertions were randomly distributed. We aimed to introduce a bias into the target site selection properties of the transposon systems by taking advantage ...

Estimating cropland latent heat flux (LE) from continental to global scales is vital to modeling crop production and managing water resources. Over the past several decades, numerous LE models were developed, such as the moderate resolution imaging spectroradiometer LE (MOD16) algorithm, revised remote sensing-based Penman-Monteith LE algorithm (RRS), the Priestley-Taylor LE algorithm of the Je...