نتایج جستجو برای: rna seq
تعداد نتایج: 256145 فیلتر نتایج به سال:
MOTIVATION Next-generation sequencing technology enables an entirely new perspective for clinical research and will speed up personalized medicine. In contrast to microarray-based approaches, RNA-Seq analysis provides a much more comprehensive and unbiased view of gene expression. Although the perspective is clear and the long-term success of this new technology obvious, bioinformatics resource...
Precision-cut lung slices (PCLS) have gained increasing interest as a model to study biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of compared with animal models. Several experimental readouts been established for use PCLS, but obtaining high-yield -quality RNA downstream analysis has remained challenging. Th...
In recent years, RNA-seq has become a very competitive alternative to microarrays. In RNA-seq experiments, the expected read count for a gene is proportional to its expression level multiplied by its transcript length. Even when two genes are expressed at the same level, differences in length will yield differing numbers of total reads. The characteristics of these RNA-seq experiments create a ...
Rapid development of next generation sequencing technology has enabled the identification of genomic alterations from short sequencing reads. There are a number of software pipelines available for calling single nucleotide variants from genomic DNA but, no comprehensive pipelines to identify, annotate and prioritize expressed SNVs (eSNVs) from non-directional paired-end RNA-Seq data. We have de...
Identification of transcription units (TUs) encoded in a bacterial genome is essential to elucidation of transcriptional regulation of the organism. To gain a detailed understanding of the dynamically composed TU structures, we have used four strand-specific RNA-seq (ssRNA-seq) datasets collected under two experimental conditions to derive the genomic TU organization of Clostridium thermocellum...
Abstract Purpose of Review Whole exome sequencing (WES) and whole-genome (WGS) are frontline approaches for the genetic diagnosis rare diseases. However, WES/WGS fails in up to 75% cases. Transcriptomics via RNA-sequencing (RNA-Seq) is a novel approach that aims increase diagnostic yield Recent Findings publications focus on success RNA-Seq increasing rates WES/WGS-negative patients 36% cases, ...
Inference of gene regulatory network structures from RNA-Seq data is challenging due to the nature of the data, as measurements take the form of counts of reads mapped to a given gene. Here we present a model for RNA-Seq time series data that applies a negative binomial distribution for the observations, and uses sparse regression with a horseshoe prior to learn a dynamic Bayesian network of in...
Haplotype phasing of genetic variants is important for clinical interpretation of the genome, population genetic analysis and functional genomic analysis of allelic activity. Here we present phASER, an accurate approach for phasing variants that are overlapped by sequencing reads, including those from RNA sequencing (RNA-seq), which often span multiple exons due to splicing. Using diverse RNA-s...
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