Equine carbonic anhydrase isozymes (CA-I and CA-II) were purified from erythrocytes by several column chromatography. Polyclonal anti-CA-I and anti-CA-II sera were produced in rabbits. Sensitive competitive enzyme-linked immunosorbent assays (ELISA) were established to determine the developmental changes in CA-I and CA-II levels in equine erythrocytes. Concentrations of CA-I and CA-II in erythr...

The two predominant scenarios for accidentally tripping a foot control are stepping into the foot control and onto the pedal, i.e., “stepping contact;” and keeping one foot on or just above the pedal at all times, i.e., “riding the pedal.” This study shows that the various designs used to minimize “stepping contact” exacerbate inadvertent activation by “riding the pedal.”