An extra copy of a rice pistil chitinase gene, termed chip, which is expressed predominantly in floral organs just before anthesis, was introduced into an economically important cultivar of rice (Oryza sativa L. cv. Koshihikari) together with the hygromycin-resistance gene (hph) as a marker gene by co-transformation. Over 100 regenerated rice plants (R0) and their self-pollinated first and seco...

Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the salicylic acid-derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyan...

Rice blast, caused by Pyricularia oryzae, is one of the most destructive rice diseases worldwide. It essential to understand population structure and race distribution P. oryzae for prevention control blast. This study collected 1584 isolates across Jiangsu province from 2003 2017 determine diversity, common dominant structures, resistance profiles using eight parameters Chinese differential cu...

Synthetic studies on bioactive compounds are described, involving phytotoxins (tobacco wildfire disease toxin tabtoxinine-beta-lactam and rice blast disease toxin pyricuol) a glutarimide antibiotic (actiketal) black vomit toxin (gizzerosine) and marine products (antifeedant pteroenone and serinol compound didemniserinolipid).

As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning ...

Thirteen polyporphic microsatellite markers suitable for population genetic structure analysis and ABC transporter and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations and rice varieties. Preliminary results indicated that each locu...

a cDNA clone of a probenazole-illdllcible gene in rice, which encoded a protein desigllated PBZI. Sequence analysis reyealed that significant homology at the amino acid level exists between the predicted PBZI protein and intrace]lular pathogenesis-related (IPR) proteins. Accumu}ation of IBZI mRNA was not induced by wollnding, but markedly induced by・inoculation with rice blast fungus. In addi・ ...

In additioll to momilactone, phytocassanes A through E (diterpene phytoalexins) were detected in rice leaves in fields suffering from rice blast. Furthermore, phytocassane accumulation was mest abundant at the edges of necrotic lesions, indicating that the phytoalexins prevent subsequent spread of the fungus from the infected site. In pot experiments the pattern of phytocassane accumulation in ...

In addition to momilactone, phytocassanes A through E (diterpene phytoalexins) were detected in rice leaves in fields suffering from rice blast. Furthermore, phytocassane accumulation was most abundant at the edges of necrotic lesions, indicating that the phytoalexins prevent subsequent spread of the fungus from the infected site. In pot experiments the pattern of phytocassane accumulation in r...

To address the effect of organic fertilizers on rice insect pests and diseases, an experiment was carried out in Cuulong Delta Rice Research Institute (CLRRI) based on the randomized complete block design with three replications. The treatments were including a combination of four animal manure compost levels (2.5, 5.0, 7.5, 10.0 tons/ha) and four organic fertilizer levels (2.5, 5.0, 7.5, 10.0 ...