نتایج جستجو برای: rflp مجازی

تعداد نتایج: 16679  

Journal: :Journal of clinical microbiology 2001
M Machouart-Dubach C Lacroix M F de Chauvin I Le Gall C Giudicelli F Lorenzo F Derouin

Dermatomycoses are very common infections caused mainly by dermatophytes. Scytalidiosis is a differential mycological diagnosis, especially in tropical and subtropical areas. Since a culture-based diagnosis takes 2 to 3 weeks, we set up a PCR-restriction fragment length polymorphism (RFLP) method for rapid discrimination of these fungi in clinical samples. The hypervariable V4 domain of the sma...

Journal: :Acta medica Iranica 2011
Fardeen Ali Malayeri Mojtaba Panjehpour Ahmad Movahedian Majid Ghaffarpour Gholam Reza Zamani Mina Hajifaraj Tabrizi Mahdi Zamani

This study determines the value of linkage analysis using six RFLP markers for carrier detection and prenatal diagnosis in familial DMD/BMD cases and their family members for the first time in the Iranian population. We studied the dystrophin gene in 33 unrelated patients with clinical diagnosis of DMD or BMD. Subsequently, we determined the rate of heterozygosity for six intragenic RFLP marker...

Journal: :Nucleic Acids Research 1990

Journal: :Nucleic Acids Research 1990

Journal: :Journal of clinical microbiology 1999
S M Marshall P L Melito D L Woodward W M Johnson F G Rodgers M R Mulvey

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified...

2017
Mojgan ARYAEIPOUR Arezoo BOZORGOMID Bahram KAZEMI Masoud BEHNIA Hakim AZIZI Mohammad Bagher ROKNI

BACKGROUND We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran. METHODS Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp....

2005
Satoshi Umemura Nobuhito Hirawa Tamio Iwamoto Satoshi Yamaguchi Yoshiyuki Toya Shunichi Kobayashi Izumi Takasaki Gen Yasuda Kouichi Tamura Masao Ishii Lu Sun William A. Pettinger

Recently, restriction fragment length polymorphism (RFLP) of aj-adrenergic receptor gene (a2-C10) digested with Bsu361 restriction enzyme has been reported in US populations. Therefore, we examined the association of this RFLP with essential hypertension by comparing the frequency of specific alleles for this gene in Japanese populations. The distribution of this RFLP was compared with that in ...

2016
MUHAMMAD SHAHZAD ZAKIA LATIF

Various molecular techniques like analysis of the amplified rDNA internal transcribed spacers (ITS), intragenic spacers and total ITS region analysis by restriction fragment length polymorphism (RFLP) has been introduced for yeast identification but there are limited databases to identify yeast species on the basis of 5.8S rDNA. In this study, twenty nine yeast strains from various sources incl...

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