نتایج جستجو برای: reversible binding
تعداد نتایج: 467454 فیلتر نتایج به سال:
In this position paper we discuss a set of problems particular to computing within limits that draws on psychological and sociological barriers. The enormity of the predicaments we are facing, global climate change and resource scarcity, together with the social, cultural and national settings in which we are facing these predicaments, are seriously hampering our possibility to address them. We...
A series of N-bridgehead tripodal receptors bearing amide functionality is reported which displays reversible binding of nitrate anions via solvent polarity controlled molecular capsule formation through a dynamic self-assembly process.
The binding of heparin to human monocytes and the monocytoid cell line U937 was characterized. Heparin binding was rapid. specific. saturable. and reversible. There was a single class of heparin binding sites. with an apparent dissociation constant of 0.19 Mmol/L and 1 .9 x 1 0 sites per cell. The binding was not dependent on the anticoagulant property of heparin. Analysis of surfaceiodinated c...
Design of a reversible biotin analog and applications in protein labeling, detection, and isolation.
To expand the applicability of the biotin-(strept)avidin system, a biotin analog with reversible binding under non-denaturing conditions has been designed, and its applications in protein labeling, detection, and isolation have been evaluated.
14-3-3 proteins have been found to regulate the plant enzyme nitrate reductase by reversible phosphoserine binding. Plant plasma-membrane H(+)-ATPases, transporters that are activated by the phytotoxin fusicoccin, appear to be regulated in a similar fashion.
A new self-folding cavitand has been assembled through metal coordination to give a thermodynamically stable ditopic receptor of nanosize dimensions which has been used in the reversible binding of di-alkylammonium and n-alkylammonium salts.
Purified bovine factor XII was radiolabeled with iodine-125 and its binding to kaolin studied. Binding was rapid and was not readily reversible upon adding unlabeled factor XII. The optimum pH for binding was in the region of pH 5-7. The isoelectric point of factor XII was pH 5.7. High concentrations of urea or increasing the ionic strength of the medium did not inhibit binding. Polyvalent macr...
To extend and improve the utility of the streptavidin-binding peptide tag (SBP-tag) in applications ranging from affinity purification to the reversible immobilization of recombinant proteins, a cysteine residue was introduced to the streptavidin mutein SAVSBPM18 and the SBP-tag to generate SAVSBPM32 and SBP(A18C), respectively. This pair of derivatives is capable of forming a disulfide bond th...
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