Liquid chromatography has been used as a means of egg protein analysis or as a method for the purification of egg proteins. Several chromatographic methods, including gel permeation, ion-exchange, reversed-phase, hydrophobic interaction, and immobilized-ligand-affinity chromatography, have been carried out for the separation or the purification of egg yolk or egg white proteins. Ion-exchange ch...

Liquid chromatography (LC) may be simply described as an analytical technique used to separate the individual components of a solution based on their relative afRnities for a liquid mobile phase and a stationary phase. There are numerous modes of LC (i.e. reversed-phase, normal-phase, ion exchange) that are commonly classiRed according to the mechanism by which separation occurs. One such mecha...

A high performance liquid chromatography method was studied for determination of 18 ?-glycyrrhetinic acid in Glycyrrhiza glabra L. (licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and UV detection at 230 nm. The...

In this paper, we present an Analytical-Numerical model for reverse gate leakage current in AlGaN/GaN high electron mobility transistors (HEMTs), which investigate the influence of hydrostatic pressure (HP) on gate-current. Salient features are incorporated occupied sub-bands interface quantum well, combined with a self-consistent solution Schrödinger and Poisson equations. Finite difference te...

Comprehensive two-dimensional liquid chromatography (LC×LC) using the Agilent 1290 Infi nity 2D-LC Solution with DAD and MS detection is applied for the analysis of citrus oils. The developed method uses normal phase liquid chromatography in the fi rst dimension and reversed-phase liquid chromatography in the second dimension. This combination provides good orthogonality and is valuable for pro...

A major problem in gel permeation chromatography (GPC) or size exclusion chromatography is non-specific binding of applied proteins to the column matrix (stationary phase). We have tested an aqueous arginine solution as the GPC mobile phase on silica-based and polymer-based columns, using mouse monoclonal antibody and recombinant human activin, interleukin-6, basic fibroblast growth factor, and...

The development of symptomless cyanosis followed the administration of drugs of the sulphonamide groups. The simultaneous administration of magnesium sulphate is to be noted as a probable etiological factor. Colebrook', in his first series of forty cases, all of whlIoim were taking magnesium sulphate, had three cases of sulph<moglobinaemia. In his second series2 of twxenty-six cases similarly t...

INTRODUCTION Chromatography is probably the most powerful analytical technique available to the modern chemist. Its power arises from its capacity to determine quantitatively many individual components present in mixture by single analytical procedure[1-2]. High-performance liquid chromatography (HPLC) is a chromatographic technique that can separate a mixture of compounds and is used in bioche...