The ability to multiplex assays in studies of complex cellular mechanisms eliminates the need for repetitive experiments, provides internal controls, and decreases waste in costs and reagents. Here we describe optimization of a multiplex assay to assess apoptosis following a palmitic acid (PA) challenge in an in vitro hypothalamic model, using both fluorescent and luminescent based assays to me...

Xanthohumol (1) is a principal prenylated chalcone found in hops. The aim of this study was to examine its influence on platelet-derived growth factor (PDGF)-BB-triggered vascular smooth muscle cell (VSMC) proliferation and migration in vitro and on experimentally induced neointima formation in vivo. Quantification of resazurin conversion indicated that 1 can inhibit PDGF-BB-induced VSMC prolif...

This study assessed the performance of a rapid, low-cost, colorimetric method, the resazurin microtitre assay (REMA) plate method, for the detection of resistance to isoniazid and rifampicin in 136 clinical isolates of Mycobacterium tuberculosis from two hospitals in Algiers. MICs were determined and the results were compared with those obtained with the conventional proportion method on Löwens...

Curcuma longa commonly known as turmeric has been used in Indian Ayurvedic medicine as a constituent to treat various disorders. It is by now clear that principal curcuminoid of turmeric; curcumin, a yellow pigment, is responsible for these beneficiary activities. The aim of the present study was to evaluate anti-mycobacterial effect of curcumin (CMN) on intracellular growth of MDR clinical iso...

This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulte...

Sesquiterpenoids are derivatives of terpenoid compound that have a diverse skeleton and broad spectrum biological activities, particularly anticancer activity. This research reported the isolation elucidation structure sesquiterpenoid from Chisocheton lasiocarpus stem bark, as well their cytotoxic Four compounds, namely eudesm-4(15)-ene-1?,6?-diol (1), allo-aromadendrane-10?,14-diol (2), allo-a...

The primary objective of this study was to assess the antibacterial sensitivity pattern of Coliforms isolates from clinical and sub-clinical mastitis to quinolones. Forty coliforms isolates from bovine clinical and subclinical mastitis were tested for antimicrobial susceptibility by measuring MIC by Resazurin dye broth microdilution method. Of the 27 E. coli isolates tested, only 7.4% of the is...

The aim of the present work was to evaluate several methods for analyzing the viability of bacteria after antibacterial photocatalytic treatment. Colony-forming unit (CFU) counting, metabolic activity assays based on resazurin and phenol red and the Live/Dead® BacLight™ bacterial viability assay (Live/Dead staining) were employed to assess photocatalytically treated Staphylococcus epidermidis a...

This article describes the development of lignocellulose-based analytical devices (LADs) for rapid bioanalysis in low-resource settings. LADs are constructed using either a single lignocellulose or a hybrid design consisting of multiple types of lignocellulose. LADs are simple, low-cost, easy to use, provide rapid response, and do not require external instrumentation during operation. Here, we ...

The effect of selenium applied as sodium selenite (Na2SeO3) on the cytotoxicity of vanadyl sulphate (VOSO4) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na2SeO3 at low doses (0.5 and 1 μM) can enhance 100 μM VOSO4-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na...