In order to determine if variations in rRNA sequence could be used for discrimination of the members of the Bacillus cereus group, we analyzed 183 16S rRNA and 74 23S rRNA sequences for all species in the B. cereus group. We also analyzed 30 gyrB sequences for B. cereus group strains with published 16S rRNA sequences. Our findings indicated that the three most common species of the B. cereus gr...

Repetitive element polymorphism-PCR (REP-PCR) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. Using the MB1 primer, REP-PCR fingerprints from 110 Bacillus strains within the "B. cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. All Bacillus anthr...

Benzene is a highly toxic organic contaminant having carcinogenic potential and a serious ground water contaminant. Refinery industry is a major source of benzene contamination in the environment. The present paper focuses on biodegradation of benzene under anaerobic condition by using enriched microbial consortium. Enriched bacterial isolates, identified by 16S rRNA sequencing technique were t...

In a study of occupational exposure to Bacillus thuringiensis, 20 exposed greenhouse workers were examined for Bacillus cereus-like bacteria in fecal samples and on biomonitoring filters. Bacteria with the following characteristics were isolated from eight individuals: intracellular crystalline inclusions characteristic of B. thuringiensis, genes for and production of B. cereus enterotoxins, an...

Five monoclonal antibodies against bacterial spores of Bacillus cereus T and Clostridium sporogenes PA3679 were developed. Two antibodies (B48 and B183) were selected for their reactivity with B. cereus T spores, two (C33 and C225) were selected for their reactivity with C. sporogenes spores, and one (D89) was selected for its reactivity with both B. cereus and C sporogenes spores. The isotypes...

To map the distribution of anthrax outbreaks and strain subtypes in Kazakhstan during 1937-2005, we combined geographic information system technology and genetic analysis by using archived cultures and data. Biochemical and genetic tests confirmed the identity of 93 archived cultures in the Kazakhstan National Culture Collection as Bacillus anthracis. Multilocus variable number tandem repeat an...

Abstract Feed is an important factor affecting the growth of cultured fish. Around 86.5% production cost aquaculture allocated for purchase feed. Good quality and efficient feed a determinant success business. Single Cell Protein (SCP) dry cell product single microorganisms. SCP generally not only protein but also free amino acids, lipids, carbohydrates, vitamins minerals. The purpose this stud...

Biocontrol of postharvest decay on apples could provide an alternative approach for chemical control. With the effort to look for effective biocontrol agents, Bacillus pumilus (B19), B. subtilis (B11), B. cereus (B16), B. subtilis (B11), B. cereus (B17), B. brevis (EN63-1) and B. licheniformis (EN74-1) were tested for their potential activity against gray mold caused by Botrytis mali on Golden ...