Since the sequencing of the human reference genome, many human disease-related genes have been discovered. However, understanding the functions of all the genes in the genome remains a challenge. The biological activities of these genes are usually investigated in model organisms such as mice and zebrafish. Large-scale mutagenesis screens to generate disruptive mutations are useful for identify...

Transcription factors that belong to the same family typically have similar, but not identical, binding specificities. As such, they can be expected to compete differentially for binding to different variants of their binding sites. Pho4 is a yeast factor whose nuclear concentration is up-regulated in low phosphate, while the related factor, Cbf1, is constitutively expressed. We constructed 16 ...

Since the sequencing of the human reference genome, many human disease-related genes have been discovered. However, understanding the functions of all the genes in the genome remains a challenge. The biological activities of these genes are usually investigated in model organisms such as mice and zebrafish. Large-scale mutagenesis screens to generate disruptive mutations are useful for identify...

The molecular mechanisms regulating the activity of the TCRα gene are required for the production of the circulating T cell repertoire. Elements of the mouse TCRα locus control region (LCR) play a role in these processes. We previously reported that TCRα LCR DNA supports a gene expression pattern that mimics proper thymus-stage, TCRα gene-like developmental regulation. It also produces transcri...

A gene-trapping vector carrying a GUS/Luciferase dual reporter gene was developed to establish an efficient and convenient screening system for T-DNA-based gene trapping in plants. A key feature of this gene trap scheme is to place two different types of reporters, luciferase (Luc) and beta-glucuronidase (GUS), as a fusion protein within a trapped gene to probe the activity of the gene. Luc is ...

Introduction Application of MR reporter genes is a new strategy in molecular imaging based on over-expression of non-toxic proteins responsible for in vivo uptake of MRI-detectable probes [1-2]. Gene-based production of contrast agents for MR cell tracking has many advantages over the standard approach using exogenous administration of superparamagnetic particles, where the imaging signal does ...

Introduction Application of MR reporter genes is a new strategy in molecular imaging based on over-expression of non-toxic proteins responsible for in vivo uptake of MRI-detectable probes [1-2]. Gene-based production of contrast agents for MR cell tracking has many advantages over the standard approach using exogenous administration of superparamagnetic particles, where the imaging signal does ...

The multicellular green alga Volvox carteri has emerged as a valuable model organism for investigating various aspects of multicellularity and cellular differentiation, photoreception phototaxis, cell division, biogenesis the extracellular matrix morphogenetic movements. While range molecular tools bioinformatics resources have been made available exploring these topics, establishment type-spec...