Molecular hydrogen is widely used by microorganisms as a source of energy. One of the best studied aerobic hydrogen oxidizers, the beta-proteobacterium Ralstonia eutropha (formerly Alcaligenes eutrophus), harbors two distinct [NiFe]-hydrogenases which catalyze the heterolytic cleavage of H2 into 2H+ and 2e-. The genes encoding the hydrogenase subunits are arranged in two large operons together ...

This paper investigates the H∞ state estimation problem for gene regulatory networks (GRNs) with timevarying delays and Markovian jumping parameters. The system output, which is used by the estimator, is assumed to be sampled and held during a sampling period. To deal with the time-varying delays, each delay function is limited to certain lower and upper bounds. By using Lyapunov-Krasovskii fun...

Application of the phylogenetic footprinting techniques to bacterial genomes generates a large number of potential regulatory sites identified upstream of orthologous genes. The next step of such analysis should be clustering of sites corresponding to one signal, that is, binding sites of one regulator. We describe an algorithm for clustering of regulatory sites and present the results of its t...

In analyzing a signal, especially a non-stationary signal, it is often necessary the desired signal to be segmented into small epochs. Segmentation can be performed by splitting the signal at time instances where signal amplitude or frequency change. In this paper, the signal is initially decomposed into signals with different frequency bands using wavelet transform. Then, fractal dimension of ...

Intracellular iron regulates gene expression by inhibiting the interaction of iron regulatory proteins (IRPs) with RNA motifs called iron-responsive elements (IREs). To assay this interaction in living cells we have developed two fluorescent IRE-based reporters that rapidly, reversibly, and specifically respond to changes in cellular iron status as well as signaling that modifies IRP activity. ...

In this paper, we study the problem of constructing a regulatory network of yeast in oxidative stress process. Discrete Dynamic System (DDS) model has been introduced in describing Gene Regulatory Networks (GRNs). However, delay effect was not taken into consideration within the model. A Time-delay DDS model composed of linear difference equations is developed to represent temporal interactions...

The phytochrome family of photoreceptors has dual molecular functions: photosensory, involving light signal perception, and regulatory, involving signal transfer to downstream transduction components. To define residues necessary specifically for the regulatory activity of phytochrome A (phyA), we undertook a genetic screen to identify Arabidopsis mutants producing wild-type levels of biologica...

Light scattering properties of human lymphocyte subpopulations selected by immunofluorescence were studied with a flow cytometer. Regulatory and B-lymphocytes showed a low orthogonal light scatter signal, whereas cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15 revealed a large orthogonal light scatter signal. Two populations in light scatter histograms could be observed with mono...

The global regulatory plan for vaccines provides a unique opportunity to develop safe and effective ones with assured quality. Methods used by regulators address challenges of new products and technologies and also increase understanding of benefits and risks of existing products. First, the laboratory-based regulatory sciences evolve correlates of immunity and safety; or improve the product ch...