an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l ...

Both color screening and regeneration are hot medium effects on charmonium production in heavy ion collisions. While they affect in an opposite way the charmonium yield, their competition in transverse dynamics bring sensitivity to the ratio of averaged transverse momentum square for charmonium, which thus can reveal more nature of the QCD medium created from the collisions. We make an excitati...

this study was conducted to investigate the effects of in vitro and regeneration of lily (lilium longiflorum) bulb scale by different plant growth regulators in b5 medium in a completely randomized design with factorial arrangement and 4 replications. treatments consisted of indole-3-acetic acid (iaa) and 2,4-d in b5 medium in four concentrations (0, 0.5, 1, 2 mg/lit) and these were combined to...

Current study was set up to determine the response of 12 Iranian winter diploid barley (Hordeum vulgare L.) cultivars to anther culture on solid FHG induction medium supplemented with 90 mg/l sucrose, 2 mg/l 2,4-D and 0.1 mg/l kinetin. For cold pretreatment each selected spike was kept in a refrigerator at 4 C for 14 days. The experimental design consisted of a completely randomized design (CRD...

An efficient regeneration system was developed for Kigelia pinnata L., a multipurpose tree belonging to the family Bignoniaceae. The nodal segments were cultured in vitro, and the optimum concentrations of plant growth regulators for callus induction were determined. The friable organogenic calli were derived from the basal cut end of the nodal segments. The highest yield of morphogenic callus ...

Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in bamboos. Ma bamboo (Dendrocalamus latiflorus Munro) is one of the most important bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and A...

An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s ...

Strategies for improvement of nerve regeneration and optimal conditions to prevent Schwann cell (SC) loss within a nerve transplant procedure are critical. The purpose of this study was to examine SC viability, which plays an important role in peripheral nerve regeneration, under various incubation conditions up to three hours. To address this issue, Schwann cell metabolic activity was determin...