background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

background: the hbv-x (hbx) protein is believed to contribute to the development of hcc. however, the molecular mechanisms involved in hbx-mediated hepatocarcinogenesis remain obscure. in this study, the effect of hepatitis b virus x gene and its protein product hbxag on expression of p53 gene in hep g2 cell line was investigated. methods: viral dna extracted from hbv-positive serum and hbx gen...

background and objectives: hbha and mtb32c have been isolated from culture supernatants of mycobacterium tu- berculosis (m. tuberculosis) and mycobacterium bovis (m. bovis) and their immunogenicity previously studies have been confirmed. in this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (mt- b32c-hbha), in producing new chimeric protein under t...

background: angiogenesis is an important process in various physiologic and pathologic states. the most significant stimulator of angiogenesis is vascular endothelial growth factor (vegf). in contrast, vasohibin1 acts as an angiogenesis inhibitor which specifically inhibits new vessels formation. the aim of the present study was cloning and expression of vasohibin1 gene in e. coli as well as pu...

human insulin-like growth factor 1 (higf-1) consists of 70 amino acids in a single chain with three intermolecular disulfide bridges possessing valuable therapeutic effects. to date, numerous variants of specifically engineered higf-1 have been produced so as to improve higf-1 biological activity, stability and stronger binding to igf-1 receptor. mecasermin is one of the modified variants with ...

Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...

The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...

Leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. Several approaches toward vaccine development against this disease have been taken. Glycoprotein (gp63) is conserved among diverse species of Leishmania and has induced immunological responses in murine models. Therefore, this glycoprotein has been considered as a second generat...