نتایج جستجو برای: recombinant chymosin

تعداد نتایج: 111276  

Journal: :The Biochemical journal 2005
A Harden A B Macallum

Hammarsten [1872, 1874, 1877] first demonstrated that the rennin action on caseinogen was specific and independent of the action of the calcium salts. His explanation was that the caseinogen molecule was split up into a large molecule (Ruase) and a smaller one (Molkeneiweiss). The "Kiise" was rendered insoluble by the presence of soluble calcium salts and formed the clot. Since then little has ...

Journal: :The Biochemical journal 1969
M L Green G Crutchfield

1. Enzymically active insoluble derivatives of chymotrypsin and rennin were prepared by coupling each enzyme to agarose as described by Porath, Axén & Ernback (1967) and rennin to aminoethylcellulose by the method of Habeeb (1967). 2. Agarose-chymotrypsin was stable over the range pH2-9, but agarose-rennin released active enzyme into solution at above pH2 and aminoethylcellulose-rennin was simi...

2013
Neelima Rajan Sharma Yudhishthir Singh Rajput Bimlesh Mann

Glycomacropeptide (GMP) is a C-terminal part (f 106-169) of kappa-casein which is released in whey during cheese making by the action of chymosin. GMP being a biologically active component has gained much attention in the past decade. It also has unique chemical and functional properties. Many of the biological properties have been ascribed to the carbohydrate moieties attached to the peptide. ...

2011
Min Sun Chao Liu

Aldosterone quantification helps evaluate the rennin-angiotensin-aldosterone system. The new bead-based multiplex platform has not been applied in aldosterone detection to achieve simultaneous measurements of multiple hormones. A new sensitive competitive bead immunoassay based on Luminex technology for detecting aldosterone in small sample volumes was developed using two-antibody coupled beads...

2016
James Burnett

Children.?By James Burnett, m.d. E. and S. Livingstone, Edinburgh. This small work is intended by the author to serve as an introduction to the subject. As such it seems to fulfil its purpose. Included in the book are separate chapters on the infectious fevers, diseases of the skin, diseases of the nose, throat and ear, and diseases of the eye. These will, doubtless, prove welcome to many. The ...

ایمانی, مهدی, توکمه‌چی, امیر, طاهری, زهرا, نوجوان, مجید,

Background: Recombinant human Calprotectin is a protein that has inhibitory effect on the growth of cancer. This substance could use as a marker or therapeutic agents for cancer. The goal of this study was to evaluate the anti-tumor property of recombinant human Calprotectin on K562 cell line. Methods: Recombinant human Calprotectin was cloned and purified in E. coli. Then, different concent...

Kamran Atarodi, Kamran Mousavi Hosseini, Mahshid Mohammadipour, Mohammad Moradi,

Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...

Journal: :iranian journal of biotechnology 2013
fatemeh shahryari masoud shams-bakhsh mohammad reza safarnejad naser safaie saeed ataei kachoiee

background: the witches’ broom disease of lime caused by candidatus phytoplasma aurantifolia, is the most devastating disease of acidian lime in the southern parts of iran. objectives: at present, no effient method has been developed for controlling the disease, therefore quarantine approaches such as early detection and subsequent eradication of infected trees is very important. toward this ...

2013
Jaewoo Yoon Takashi Kikuma Jun-ichi Maruyama Katsuhiko Kitamoto

Aspergillus oryzae has been utilized as a host for heterologous protein production because of its high protein secretory capacity and food-safety properties. However, A. oryzae often produces lower-than-expected yields of target heterologous proteins due to various underlying mechanisms, including degradation processes such as autophagy, which may be a significant bottleneck for protein product...

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