Chorismate mutase acts at the first branch-point of aromatic amino acid biosynthesis and catalyzes the conversion of chorismate to prephenate. The results of molecular dynamics simulations of the substrate in solution and in the active site of chorismate mutase are reported. Two nonreactive conformers of chorismate are found to be more stable than the reactive pseudodiaxial chair conformer in s...

A fascinating group of enzymes has been shown to possess multiple active sites connected by intramolecular tunnels for the passage of reactive intermediates from the site of production to the site of utilization. In most of the examples studied to date, the binding of substrates at one active site enhances the formation of a reaction intermediate at an adjacent active site. The most common inte...

A web site presents a graph–like spatial structure composed of pages connected by hyperlinks. This structure may represent an environment in which situated agents associated to visitors of the web site are positioned and moved in order to monitor their navigation. This paper presents a heterogeneous multiagent system supporting the collection of information related to user’s behaviour in a web ...

Reaction thermodynamics and potential energy surfaces are calculated using density functional methods to investigate possible reactive Cu/O(2) species for H-atom abstraction in peptidylglycine alpha-hydroxylating monooxygenase (PHM), which has a noncoupled binuclear Cu active site. Two possible mononuclear Cu/O(2) species have been evaluated, the 2-electron reduced Cu(II)(M)-OOH intermediate an...

Introduction: Hamartomas is a neoplasms composed of mature tissue elements from the affected site with disproportion between their components. Although lingual hamartomas are traditionally infrequent in the head and neck, a significant number of case reports with this disorder in infancy are arising from the literature.   Case Report:</em...

The Mustard Trypsin Inhibitor (MSI) family is a small family of plant protease inhibitors so far only found in Brassicaceae. Using a phage display selection, MTI-2 (Mustard Trypsin Inhibitor 2) mutants were detected and analysed for their biochemical characteristics. Retromutants of the selected MTI-2 proteins were constructed and expressed in the Pichia pastoris system. The recombinant protein...

sepsis is a significant health problem with an estimated 750,000 new cases in the usa annually. it is also the third leading cause of death in developed countries, equaling the number of fatalities from acute myocardial infarction. the high sepsis-related mortalities mean there is an urgent need to improve the diagnosis and management of sepsis patients. the aim of this study was the evaluation...

Traumatic brain injury (TBI) is a disruption in the brain functions following a head trauma. Cell therapy may provide a promising treatment for TBI. Human neural stem cells cultured in self-assembling peptide scaffolds have been proposed as a potential novel method for cell replacement treatment after TBI. In the present study, we accessed the effects of human neural stem/progenitor cells (hNS/...

Cloning and expression of the L-phenylalanine dehydrogenase gene, from B. sphaericus in E. coli were done. The gene was cloned in the vector pET16b and transformed into E. coli BL21 (DE3). The functional form of the L-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, Re...

Introduction: Choristoma is a non-neoplastic growth of normal tissues in non-indigenous site of origin. Osseous Choristoma is an ectopic bone formation which is a rare entity with 72 cases has been reported in the tongue, 15 cases in the buccal mucosa, 1 case in the lower lip.   Case Report: A 43 year old male patient experienced a mild, intermittent, dull aching type in...