Background Brazil is the world’s leading ethanol producer and exporter. Due to increasing fuel demands, ethanol production has escalated in recent years. Vinasse is generated as a byproduct of the ethanol industry and is mainly used as fertilizer. The excessive usage of vinasse in soil leads to groundwater contamination [1]. The objective of this project is to develop a fermentation process wit...

Poly(3-hydroxybutyrate) (PHB) granules are organelle-like multienzyme-polymer complexes (carbonosomes) and are widespread storage compounds in prokaryotes. The interaction of three PHB granule-bound proteins (PHB synthase PhaC1, phasin PhaP5, and PHB/DNA binding protein PhaM) was studied in vivo by bimolecular fluorescence complementation (BiFC) microscopy in Ralstonia eutropha. To this end, a ...

Ralstonia metallidurans, formerly known as Alcaligenes eutrophus and thereafter as Ralstonia eutropha, is a beta-Proteobacterium colonizing industrial sediments, soils or wastes with a high content of heavy metals. The type strain CH34 carries two large plasmids (pMOL28 and pMOL30) bearing a variety of genes for metal resistance. A chronological overview describes the progress made in the knowl...

Pseudomonas oleovorans NCIMB 6576 and Ralstonia eutropha 10442 were used for the production of Polyhydroxyalkanoates (PHA) from industrial waste cooking oils, bacteria cultured on tryptone soya broth (TSB) Tryptone agar (TSA). P. NCIMB6576 gave a better percentage PHB yield (8.2%) with PS oil as carbon source compared to 6.45% TS oil. However, very low (0.64%) was recorded when grown TSB withou...

Two phenol-degrading organisms, Ralstonia eutropha TCP and a Pseudomonas aeruginosa strain, were studied for their co-metabolic capabilities towards chlorophenol (CP) isomers at different substitution grade. R. eutropha TCP resting cells, grown on different substrates, oxidised to varying degrees and regardless the growth substrate, some CP, while the dechlorinating activity/ies of the strain w...

In a previous study, the essential role of 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase acyl-CoA dehydrogenase (Acd) in Advenella mimigardefordensis strain DPN7(T) (AcdDPN7) during degradation of 3,3'-dithiodipropionic acid (DTDP) was elucidated. DTDP is a sulfur-containing precursor substrate for biosynthesis of polythioesters (PTEs). AcdDPN7 showed high amino acid sequence similarity t...