In this Letter, we report on the efficient generation of electrons from metals using multiphoton photoemission by use of nanostructured plasmonic surfaces to trap, localize, and enhance optical fields. The plasmonic surface increases absorption over normal metals by more than an order of magnitude, and due to the localization of fields, this results in over 6 orders of magnitude increase in eff...

We present an algorithm for simultaneous reconstruction of optical parameters, quantum yield, and lifetime in turbid media with embedded fluorescent inclusions. This algorithm is designed in the Fourier domain as an iterative solution of a system of differential equations of the Helmholtz type and does not involve full ill-conditioned matrix computations. The approach is based on allowing the u...

Fluorescent cross-linked nanoparticles with variable fluorophore loading amounts, locations, and particle sizes were synthesized from sequential one-pot functionalization/cross-linking of block copolymer micelles with amine-terminated dye and cross-linker molecules, via reductive amination and amidation. The fluorescence quantum yield and brightness of these nanoparticles were evaluated by stea...

Regulation of nonradiative dissipation of absorbed light energy in PSII is an indispensab]e process to ayoid photoinhibition in plants. To dissect molecular mechanisms of the regulation, we identified Arabidopsis mutants with reduced quenching of Chl fluorescence using a fiuorescence imaging system. By analyses of Chl fluorescence induction pattern in the light and quantum yield of both photosy...

We have measured the radical pair quantum yield of reaction centers of photosystem II of green plants and of the purple bacterium Rhodobacter sphaeroides as a function of excitation density using sub-picosecond pulses at 77 K. The saturation behavior of both RCs can be described consistently by taking into account stimulated emission and the geometry of the polarized (magic angle) laser experim...

A series of π conjugated naphthalimide derivatives having an imide group as an acceptor conjugated with a methoxy arylethynyl or a methoxyphenyl triazole as a donor were prepared by Sonogashira coupling or "click" chemistry. Their photophysical properties were investigated by steady state and time resolved fluorescence spectroscopy and modelled by TD-DFT calculations. Compound Naphth-AlkyneOMe ...

The main challenge in developing new wavelength-specific photolabile protecting groups is the rigorous control of the photolysis rate. This rate is controlled by two factors: the chromophore absorbance and the reaction quantum yield. Fine-tuning the properties by changing substituents or structural features is difficult, because both factors are independently affected. By the use of the kinetic...

We have explored amyloid formation using poly(amino acid) model systems in which differences in peptide secondary structure and hydrophobicity can be introduced in a controlled manner. We show that an environmentally sensitive fluorescent dye, dapoxyl, is able to identify β-sheet structure and hydrophobic surfaces, structural features likely to be related to toxicity, as a result of changes in ...

Detection of dye-labeled nucleic acids via fluorescence reporting has become a routine technique in molecular biology laboratories. Given this, it is important to note that the quantum yield of fluorophores attached to nucleic acids is dependent upon a number of factors. One of these is the choice of the base that lies adjacent to the fluorescent molecule. Fluorescence quenching by an adjacent ...

A simple low temperature colloid-chemical synthetic method is reported for size controlled synthesis of hydrophobic silicon nanoparticles in the 1-10 nm range. These silicon nanoparticles show size dependent tunable visible emission from blue to red with fluorescence quantum yield in the range of 6-13%. These silicon nanoparticles can be subjected to extensive surface chemistry without signific...