نتایج جستجو برای: quantitative competitor pcr

تعداد نتایج: 463288  

In this work, the removal of copper from an aqueous solution by granular activated carbon (GAC) in the presence of competitor ions was studied. A batch adsorption was carried out and different parameters such as pH, contact time, initial copper concentration and competitor ions concentration were changed to determine the optimum conditions for adsorption. The optimum pH required for maximum ads...

Journal: :BioTechniques 1997
M Fille J D Shanley J Aslanzadeh

One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...

1998
Jean Peccoud Christine Jacob

Current quantitative PCR protocols are only indicative of the relative quantity of a target sequence to a standard since no means of estimating the amplification rate is available as yet. The variability of PCR performed on isolated cells has already been reported by several authors, but it could not be extensively studied due to lack of a system for doing kinetic data acquisition and of statis...

2004
Val A. Hooper Sid L. Huff Peter C. Thirkell

This study explores the alignment between IT/IS and marketing from a strategic perspective. The heads of IT and marketing of eighteen large New Zealand companies were interviewed, and their assessments of alignment rated according to two constructs: strategic orientation, and market orientation. The strategic orientation dimensions were aggressiveness, analysis, defensiveness, futurity, proacti...

Journal: :advances in environmental technology 0
saeed almohammadi department of chemical engineering, mahshahr branch, islamic azad university, mahshahr, iran masoomeh mirzaei department of chemical engineering, mahshahr branch, islamic azad university, mahshahr, iran

in this work, the removal of copper from an aqueous solution by granular activated carbon (gac) in the presence of competitor ions was studied. a batch adsorption was carried out and different parameters such as ph, contact time, initial copper concentration and competitor ions concentration were changed to determine the optimum conditions for adsorption. the optimum ph required for maximum ads...

Journal: :Clinical chemistry 1994
R Sestini C Orlando L Zentilin S Gelmini P Pinzani S Bianchi C Selli M Giacca M Pazzagli

We present an original application of competitive polymerase chain reaction (PCR) for measuring oncogene amplification in DNA from human tumors by simultaneous PCR amplification of genomic DNA with fixed amounts of an internal standard (competitor DNA). Competitors share the same sequence as the target genes but contain an additional 15- to 20-base-pair insert, which allows resolution of the am...

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