نتایج جستجو برای: ptz57r cloning vector

تعداد نتایج: 249716  

Background & objective:  Tuberculosis (TB) remains a major cause of death around the world. Bacillus Calmette Guérin (BCG) is the only vaccine used in TB prevention that has a protective effect in children, but its effectiveness declines in adults. Design and development of new vaccines is the most effective way against TB. The aim of this study was to design and construc...

Journal: :Nucleic acids research 2001
V N Noskov M Koriabine G Solomon M Randolph J C Barrett S H Leem L Stubbs N Kouprina V Larionov

The transformation-associated recombination (TAR) cloning technique allows selective and accurate isolation of chromosomal regions and genes from complex genomes. The technique is based on in vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or hooks, to the gene of interest. The recombination occurs during transformation of yeast spheroplasts that ...

Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...

Journal: :Applied and environmental microbiology 2014
Karina Klevanskaa Nadja Bier Kerstin Stingl Eckhard Strauch Stefan Hertwig

An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of ...

2014
Ming V. Li Dip Shukla Brian H. Rhodes Anjali Lall Jingmin Shu Branden S. Moriarity David A. Largaespada

Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in the design and construction of complex DNA molecules by standardizing all DNA elements and cloning reactions. Our system, named HomeRun Vector As...

Journal: :Protein expression and purification 2014
Mats A Holmberg Naveen Kumar Chandappa Gowda Claes Andréasson

Production of recombinant proteins is the starting point for biochemical and biophysical analyses and requires methodology to efficiently proceed from gene sequence to purified protein. While optimized strategies for the efficient cloning of single-gene fragments for bacterial expression is available, efficient multiple DNA fragment cloning still presents a challenge. To facilitate this step, w...

Journal: :Nucleic acids research 1996
F Boë J M Masson

Repetitive DNA sequences play an important role in biology, especially at the level of eucaryotic gene transcription control. Their construction, assembly and cloning by conventional means or by PCR derived methods is usually very cumbersome. This method is based on the association of a small DNA tag with the sequence to be polymerised. The multimeric sequence is constructed in a stepwise manne...

Journal: :Applied and environmental microbiology 2012
Jae-Yeon Jeong Hyung-Soon Yim Ji-Young Ryu Hyun Sook Lee Jung-Hyun Lee Dong-Seung Seen Sung Gyun Kang

We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...

2010
Ronald Godiska David Mead Vinay Dhodda Chengcang Wu Rebecca Hochstein Attila Karsi Karen Usdin Ali Entezam Nikolai Ravin

Despite recent advances in sequencing, complete finishing of large genomes and analysis of novel proteins they encode typically require cloning of specific regions. However, many of these fragments are extremely difficult to clone in current vectors. Superhelical stress in circular plasmids can generate secondary structures that are substrates for deletion, particularly in regions that contain ...

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